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GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin

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Abstract

This work investigated the effect of gallium arsenide (GaAs) irradiation (power: 5 mW; intensity: 77.14 mW/cm2, spot: 0.07 cm2) on regenerating skeletal muscles damaged by crotoxin (CTX). Male C57Bl6 mice were divided into six groups (n = 5 each): control, treated only with laser at doses of 1.5 J or 3 J, CTX-injured and, CTX-injured and treated with laser at doses of 1.5 J or 3 J. The injured groups received a CTX injection into the tibialis anterior (TA) muscle. After 3 days, TA muscles were submitted to GaAs irradiation at doses of 1.5 or 3 J (once a day, during 5 days) and were killed on the eighth day. Muscle histological sections were stained with hematoxylin and eosin (H&E) in order to determine the myofiber cross-sectional area (CSA), the previously injured muscle area (PIMA) and the area density of connective tissue. The gene expression of MyoD and myogenin was detected by real-time PCR. GaAs laser at a dose of 3 J, but not 1.5 J, significantly increased the CSA of regenerating myofibers and reduced the PIMA and the area density of intramuscular connective tissue of CTX-injured muscles. MyoD gene expression increased in the injured group treated with GaAs laser at a dose of 1.5 J. The CTX-injured, 3-J GaAs laser-treated, and the CTX-injured and treated with 3-J laser groups showed an increase in myogenin gene expression when compared to the control group. Our results suggest that GaAs laser treatment at a dose of 3 J improves skeletal muscle regeneration by accelerating the recovery of myofiber mass.

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Acknowledgments

The authors dedicate this work to the co-author and great friend Dr. Cláudio A. Toledo, who passed away recently. This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). LHS, MTS, and TCC received scholarships from FAPESP (grants# 2009/50198-6, 2009/54240-7 and 2007/56879-0, respectively).

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The authors have no conflicts of interest.

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Authors and Affiliations

  1. Department of Anatomy, Biomedical Sciences Institute, University of São Paulo, Lineu Prestes Av., 2415, 05508-000, São Paulo, SP, Brazil

    Lucila H. Silva, Meiricris T. Silva, Talita C. Conte & Elen H. Miyabara

  2. Neuroscience Research Nucleus, University of São Paulo City, São Paulo, SP, Brazil

    Rita M. Gutierrez, Cláudio A. Toledo & Richard E. Liebano

  3. School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, SP, Brazil

    Marcelo S. Aoki

Authors
  1. Lucila H. Silva
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  2. Meiricris T. Silva
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  3. Rita M. Gutierrez
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  4. Talita C. Conte
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  5. Cláudio A. Toledo
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  6. Marcelo S. Aoki
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  7. Richard E. Liebano
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  8. Elen H. Miyabara
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Correspondence to Elen H. Miyabara.

Additional information

Lucila H. Silva and Meiricris T. Silva contributed equally to this work.

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Silva, L.H., Silva, M.T., Gutierrez, R.M. et al. GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin. Lasers Med Sci 27, 993–1000 (2012). https://doi.org/10.1007/s10103-011-1031-x

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  • DOI: https://doi.org/10.1007/s10103-011-1031-x

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