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Evaluation of the furcation area permeability of deciduous molars treated by neodymium:yttrium–aluminum–garnet laser or adhesive

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Abstract

The purpose of this in vitro study was to compare the effects of a self-etch adhesive system and neodymium:yttrium–aluminum–garnet (Nd:YAG) laser application on the dentinal permeability of the furcation area of primary molars. After endodontic access, 39 extracted human deciduous molars were divided into three groups: control group (CG), no treatment; adhesive group (AG), self-etching adhesive was applied to the furcation area; laser group (LG), specimens were irradiated with Nd:YAG laser. To evaluate dentin permeability of the furcation area, we immersed the specimens in 0.5 % methylene blue dye for 4 h. Then, they were longitudinally sectioned into two halves and photographed. The images were analyzed by two qualified evaluators using TpsDig software to calculate the percentage of the dye penetration area in comparison with the total furcation area. Additional analyses by scanning electron microscopy (SEM) were performed. The analysis of variance (ANOVA), complemented by Student’s t-test, showed that mean dye penetration in the LG was statistically significant lower than that in all the other groups (P < 0.05). The SEM analysis showed mostly dentinal tubules obliterated by smear layer in the CG; in the AG the smear layer was modified by the adhesive, and, in the LG, melted surfaces were observed. It can be concluded that the Nd:YAG laser was capable of reducing the dentinal permeability of the furcation area of deciduous molars.

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Acknowledgments

The authors wish to express their gratitude to: LELO (Special Laboratory of Lasers in Dentistry), University of São Paulo, Brazil and FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo, grant 2007/55487-0), for providing the Nd:YAG laser; to Professor Victor E. Arana-Chavez, from the Oral Biology Laboratory, Department of Dental Materials, School of Dentistry, University of São Paulo, Brazil, for his assistance in the preparation of the samples for the scanning electron microscope, and to PhD student Nilton Azambuja, Department of Restorative Dentistry, for his assistance in taking the electron micrographs.

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Authors and Affiliations

  1. Department of Restorative Dentistry, School of Dentistry, University of São Paulo (USP), Av. Prof. Lineu Prestes, 2227, São Paulo, SP, 005508-000, Brazil

    Camila A. B. Guglielmi & Tais Scaramucci

  2. Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

    Karen Müller Ramalho

  3. Department of Pediatric Dentistry, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil

    Sandra Regina Echeverria P. da Silva & Sérgio Luiz Pinheiro

  4. Universidade Camilo Castelo Branco (Unicastelo), São Paulo, SP, Brazil

    Sandra Regina Echeverria P. da Silva

  5. Department of Pediatric Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil

    José Carlos P. Imparato

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  1. Camila A. B. Guglielmi
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  2. Karen Müller Ramalho
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  3. Tais Scaramucci
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  6. Sérgio Luiz Pinheiro
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Correspondence to Camila A. B. Guglielmi.

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Guglielmi, C.A.B., Müller Ramalho, K., Scaramucci, T. et al. Evaluation of the furcation area permeability of deciduous molars treated by neodymium:yttrium–aluminum–garnet laser or adhesive. Lasers Med Sci 25, 873–880 (2010). https://doi.org/10.1007/s10103-009-0730-z

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  • DOI: https://doi.org/10.1007/s10103-009-0730-z

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