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Using MALDI-TOF MS typing method to decipher outbreak: the case of Staphylococcus saprophyticus causing urinary tract infections (UTIs) in Marseille, France

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Abstract

Staphylococcus saprophyticus is one of the leading causes of urinary tract infections (UTI). In December 2014, our surveillance system identified an abnormal increase in S. saprophyticus causing UTIs in four university hospitals in Marseille, indicating a suspected community S. saprophyticus UTI outbreak. This was detected by our surveillance system BALYSES (Bacterial real-time Laboratory-based Surveillance System). S. saprophyticus/ Escherichia coli UTI ratio increased three-fold from 0.0084 in 2002 to 0.025 in December 2015 in Marseille with an abnormal peak in December 2014, and with an annual estimated ratio trend of 5.10−6 (p-value < 10−3). Matrix-Assisted Laser Desorption Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis of strains was used to analyse strains cluster expansion, comparing strains from Marseille to those from Nice during the same period. MALDI-TOF MS spectral analysis revealed a geographical restricted clonal expansion of the strains clusters in Marseille as compared to Nice. Our finding suggests (i) a geographically restricted expansion of a specific S. saprophyticus strain clusters circulating in Marseille, and (ii) MALDI-TOF MS can be used as a cost-effective tool to investigate an outbreak.

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Authors and Affiliations

  1. URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU-Méditerranée Infection, Aix-Marseille Université, 19–21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France

    K. D. Mlaga, G. Dubourg, C. Abat, H. Chaudet, S. M. Diene, D. Raoult & J.-M. Rolain

  2. Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, University Hospital Centre Timone, Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique - Hôpitaux de Marseille, Marseille, France

    G. Dubourg, C. Abat, H. Chaudet, S. M. Diene, D. Raoult & J.-M. Rolain

  3. Department of Bacteriology at Nice Academic Hospital, Nice Medical University, Nice, France

    L. Lotte & R. Ruimy

  4. INSERM U1065 (C3M), Bacterial Toxins in Host Pathogen Interactions, C3M, Bâtiment Universitaire Archimed, Nice, France

    R. Ruimy

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  1. K. D. Mlaga
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  2. G. Dubourg
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  3. C. Abat
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  4. H. Chaudet
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  5. L. Lotte
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  6. S. M. Diene
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  7. D. Raoult
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  9. J.-M. Rolain
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Correspondence to J.-M. Rolain.

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This work was funded by IHU Méditerranée Infection.

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Mlaga, K.D., Dubourg, G., Abat, C. et al. Using MALDI-TOF MS typing method to decipher outbreak: the case of Staphylococcus saprophyticus causing urinary tract infections (UTIs) in Marseille, France. Eur J Clin Microbiol Infect Dis 36, 2371–2377 (2017). https://doi.org/10.1007/s10096-017-3069-6

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  • DOI: https://doi.org/10.1007/s10096-017-3069-6

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