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Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen

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Abstract

The purpose of this study was to characterise methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 2005 at the university hospitals of Debrecen, Hungary. Three hundred and thirty-nine MRSA strains were isolated from 102 patients at 18 different clinics. Their sensitivity to oxacillin and ten other antibiotics was determined. For genotypic analysis, phage typing and pulsed-field gel electrophoresis (PFGE) was performed. The rate of MRSA strains increased to 7.2% in 2005, especially at the clinics of surgery, pulmonology and paediatrics. No vancomycin- or teicoplanin-resistant strains were found. The resistance to erythromycin, clindamycin and ciprofloxacin was nearly 100% and multi-resistance was very frequent. Fifty-eight percent of the isolates belonged to mixed phage types and 8% was non-typable. One PFGE clone contained 58.2% of all strains and two further major clones were found at a separately located clinical block, indicating intra-hospital spread. We can conclude that MRSA exhibits an increasing nosocomial problem also in Hungary.

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Acknowledgement

We would like to thank Mrs. Erzsébet Papp Falusi and Zsolt Hartman for their skilful assistance. We are especially grateful to Dr. Judit Horváth for performing the phage typing.

This work was presented in part at the Annual Meeting of the Hungarian Society for Microbiology, Keszthely, Hungary, October 2006.

This work was supported by the Hungarian Ministry of Health, Social and Family Affairs, grant no. 221/2003, and by the National Scientific Research Fund (OTKA), grant nos. M36764, T46186 and F61665.

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Authors and Affiliations

  1. Department of Medical Microbiology, Medical and Health Centre, University of Debrecen, P. O. B. 17, 4012, Debrecen, Hungary

    J. Szabó, Z. Dombrádi & J. Kónya

  2. Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary

    O. Dobay, K. Nagy & F. Rozgonyi

  3. Division of Hospital Hygiene, Medical and Health Centre, University of Debrecen, Debrecen, Hungary

    P. Orosi

  4. Microbiology Laboratory, Department of Dermatology, Venerology and Dermatooncology, Semmelweis University, Budapest, Hungary

    F. Rozgonyi

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  1. J. Szabó
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  2. Z. Dombrádi
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  3. O. Dobay
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  4. P. Orosi
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  5. J. Kónya
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  6. K. Nagy
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  7. F. Rozgonyi
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Correspondence to J. Szabó.

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Zsuzsa Dombrádi contributed equally to the first authorship.

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Szabó, J., Dombrádi, Z., Dobay, O. et al. Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen. Eur J Clin Microbiol Infect Dis 28, 129–136 (2009). https://doi.org/10.1007/s10096-008-0588-1

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