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In vitro and in vivo anti-inflammatory activities of ethanolic extract of Sargassum confusum

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Abstract

This study was to investigate the anti-inflammatory activity of ethanolic extract of Sargassum confusum (SCEE). LPS is recognized by TLR4 on the macrophage surface, which activates the NF-κB and MAPK signaling pathways, increasing the expression of inflammatory cytokine like interleukin-6 (IL-6), IL-1β, and tumor necrosis factor (TNF)-α and inflammatory mediators such as nitric oxide (NO) and cyclooxygenase-2 (COX-2). The anti-inflammatory effect of SCEE (0, 1, 10, 50 μg/mL) was identified using LPS stimulated RAW 264.7 macrophages. As a result, it was confirmed that inflammatory factors were suppressed by inhibiting expression of COX-2 and iNOS without any cytotoxicity. In addition, phosphorylation of ERK, JNK and NF-κB p65, transcription factors, were inhibited by SCEE does-dependent manner. These results suggest that SCEE has an anti-inflammatory effect and can be used as a material for health functional foods to prevent inflammatory diseases.

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Acknowledgements

This research was supported by Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (20210656).

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Authors and Affiliations

  1. Department of Food Science & Technology, Pukyong National University, Busan, 48513, Korea

    Ga Eun Woo, Min Ji Kim & Dong-Hyun Ahn

  2. Institute of Food Science, Pukyong National University, Busan, 48513, Korea

    Dong-Hyun Ahn

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  1. Ga Eun Woo
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  2. Min Ji Kim
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Correspondence to Dong-Hyun Ahn.

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Woo, G.E., Kim, M.J. & Ahn, DH. In vitro and in vivo anti-inflammatory activities of ethanolic extract of Sargassum confusum. Food Sci Biotechnol 33, 181–190 (2024). https://doi.org/10.1007/s10068-023-01439-2

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