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Mechanism of gastrointestinal adaptability and antioxidant function of infant-derived Lactobacillus plantarum BF_15 through genomics

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Abstract

Lactobacillus plantarum is an essential probiotic in the human gastrointestinal tract. L. plantarum BF_15, a functional probiotic isolated from the feces of breast-fed infants, has been reported in many in vitro and in vivo studies with strong gastrointestinal adaptability and outstanding anti-oxidative activities. Therefore, the whole genome of L. plantarum BF_15 was sequenced. Several genes, encoding the gastrointestinal adaptability-related proteins, were identified, including genes related to gastrointestinal environment-induced stress resistance, adhesive performance, and ability to transport and metabolize resistant starch and oligosaccharides. Genes related to alleviating oxidative stress were also found. Further functional verification was carried out by RT-qPCR on the 10 and 12 key adhesion and antioxidant genes. Overall, this study might provide a critical basis for L. plantarum BF_15 as a potential candidate for probiotics.

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Acknowledgements

This research was funded by the Key Research and Development Program of Hebei Province (Grant No. 19227134D, 20327112D), the Science and Technology Research Projects of Colleges and Universities in Hebei Province (Grant No. ZD2021059), the Food Processing Discipline Group of Hebei Agricultural University (Grant No. 2022-08), the Doctoral Fund of Baoding University (Grant No. 2021Z02), and the Research Foundation for the Introduced Talents of Hebei Agricultural University (Grant No. YJ2021035).

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Author notes

  1. Xinyu Wang, Na Zhang, and Dongyao Li have contributed equally to this work.

Authors and Affiliations

  1. College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei, 071000, China

    Xinyu Wang, Dongyao Li, Chen Li & Hongtao Tian

  2. College of Biochemistry and Environmental Engineering, Baoding University, Baoding, Hebei, 071000, China

    Na Zhang

  3. New Hope Tensun (Hebei) Dairy Co. Ltd, Baoding, Hebei, 071000, China

    Miaoshu Wang

  4. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding, Hebei, 071000, China

    Hongtao Tian

  5. Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding, Hebei, 071000, China

    Na Zhang, Dongyao Li, Miaoshu Wang, Chen Li & Hongtao Tian

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  1. Xinyu Wang
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Wang, X., Zhang, N., Li, D. et al. Mechanism of gastrointestinal adaptability and antioxidant function of infant-derived Lactobacillus plantarum BF_15 through genomics. Food Sci Biotechnol 31, 1451–1462 (2022). https://doi.org/10.1007/s10068-022-01132-w

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  • DOI: https://doi.org/10.1007/s10068-022-01132-w

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