Abstract
Campylobacter spp. are major causes of gastrointestinal infections worldwide, and are commonly identified using modified-charcoal-cefoperazone-deoxycholate agar (mCCDA). However, the efficacy of this screening technique is often hindered by overgrowth of competing flora, such as extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. Thus, in the present study we supplemented mCCDA with a recently developed ESBL inhibitor, avibactam (A-mCCDA). We inoculated mCCDA and A-mCCDA plates with 25 strains each of Campylobacter spp. and ESBL-producing E. coli, and thereby determined that the optimum avibactam concentration required to inhibit ESBL-producing E. coli was 0.0625 mg/L. At this concentration, a significantly higher proportion of Campylobacter spp. was isolated using A-mCCDA compared to that using mCCDA (P < 0.05). Thus, the results of the present study support the use of A-mCCDA to improve current Campylobacter screening methods.
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Acknowledgements
This work was supported by a National Research Foundation of Korea grant funded by the Korean government (MSIP) (2018R1A2A2A14021671), and by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Research Center Support Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (716002-7). The authors thank Drs Young-Ji Kim, Jung-Whan Chon, Dongryeoul Bae, Dong-Hyeon Kim, Hyunsook Kim, and Kwang-Young Song for their valuable advises in designing experiments and technical assistance.
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Kim, B., Seo, KH. Development of a selective media for detecting Campylobacter spp. in chicken carcasses using avibactam supplemented mCCDA. Food Sci Biotechnol 29, 1159–1163 (2020). https://doi.org/10.1007/s10068-020-00759-x
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DOI: https://doi.org/10.1007/s10068-020-00759-x