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DNA profiling of Leuconostoc mesenteroides strains isolated from fermented foods and farm produce in Korea by repetitive-element PCR

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Abstract

Lactic acid bacteria are known for their preservative effects on food products like meat and sausage. Since they are related to humans, these bacteria require proper characterization and identification among various other bacteria in the surroundings. For their identification, several typing methods have already been applied of which the genotyping methods provide reproducible and unambiguous results. In this study, PCR-based method called repetitive element PCR was used for typing 37 Leuconostoc mesenteroides with three primers, REP, ERIC, and (GTG)5, annealing to repetitive sequences present in the bacterial genome. Different fingerprints were obtained for the isolates showing distinguishing profiles. Further phylogenetic analysis was performed using UPGMA method of clustering which provided proper identification with genetic relatedness of all the isolates. It was finally observed that, out of the three primers used, (GTG)5 discriminated the strains precisely than the other two.

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Acknowledgements

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA; Grant Number: 314073-03-2-HD040).

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120, Korea

    Jasmine Kaur, Sulhee Lee, Anshul Sharma & Young-Seo Park

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  1. Jasmine Kaur
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  2. Sulhee Lee
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  3. Anshul Sharma
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  4. Young-Seo Park
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Correspondence to Young-Seo Park.

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Kaur, J., Lee, S., Sharma, A. et al. DNA profiling of Leuconostoc mesenteroides strains isolated from fermented foods and farm produce in Korea by repetitive-element PCR. Food Sci Biotechnol 26, 1667–1673 (2017). https://doi.org/10.1007/s10068-017-0189-9

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  • DOI: https://doi.org/10.1007/s10068-017-0189-9

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