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A new high-molecular-weight glutenin subunit from the slovak wheat (Triticum aestivum L.) cultivar ‘Trebišovská 76’

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Abstract

Historical and local varieties are an important source of genetic diversity in connection with the wheat baking quality. By analysis of DNA from the coding part of the genome of the Slovak regional wheat (Triticum aestivum L.) variety ‘Trebišovská 76’, differences in the Dy subunit composition coded by locus Glu-1D were found. Comparing the newly found sequence with a generally used 1Dy12 (GenBank accession no. X03041) sequence, existence of a new high-molecular-weight glutenin subunit, which was marked as 1Dy12* (GenBank accession no. EU266533) was confirmed. The molecular weight of probable protein coded by 1Dy12* subunit reaches 72.35 kDa comparing to the 1Dy12, which reaches 68.71 kDa. This difference is not likely to distinguish by SDS-PAGE. The easiest way for cereal genotyping according to baking quality is proteomic analysis. More exact and suitable method for genotype identification appears to be marker assisted selection using PCR.

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Authors and Affiliations

  1. Plant Production Research Center, SK-921 68, Piestany, Slovak Republic

    Daniel Mihálik, Katarína Ondreičková, Marcela Gubišová, Jozef Gubiš, Edita Gregová, Ivana Dokupilová & Ján Kraic

  2. Institute of High Mountain Biology, University of Žilina, Tatranská Javorina 7, SK-059 56, Tatranská Javorina, Slovak Republic

    Daniel Mihálik

  3. Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, SK-949 74, Nitra, Slovak Republic

    Ľubica Nogová & Marcela Gubišová

  4. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, SK-842 15, Bratislava, Slovak Republic

    Roman Drška

  5. Department of Biotechnology, University of SS. Cyril and Methodius in Trnava, SK-917 01, Trnava, Slovak Republic

    Ján Kraic

Authors
  1. Daniel Mihálik
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  2. Ľubica Nogová
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  3. Katarína Ondreičková
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  4. Marcela Gubišová
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  5. Jozef Gubiš
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  6. Edita Gregová
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  7. Ivana Dokupilová
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  8. Roman Drška
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  9. Ján Kraic
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Correspondence to Ján Kraic.

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Mihálik, D., Nogová, Ľ., Ondreičková, K. et al. A new high-molecular-weight glutenin subunit from the slovak wheat (Triticum aestivum L.) cultivar ‘Trebišovská 76’. Food Sci Biotechnol 22 (Suppl 1), 33–37 (2013). https://doi.org/10.1007/s10068-013-0045-5

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  • DOI: https://doi.org/10.1007/s10068-013-0045-5

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