Abstract
Food spoilage is a major problem faced by consumers across the globe. As an enzyme that degrades DNA, DNase production on fish tissue seemed likely to aid in fish spoilage. Based on physical characteristics, bacteria producing extracellular DNase were isolated on selective media. 16S rDNA sequences were obtained identifying isolates as bacteria belonging to Aeromonas spp., Serratia spp., Shewanella spp., and Rahnella spp. Aeromonas spp. were the predominant bacteria isolated in this study; this statistically suggests that Aeromonas spp. are dominant in DNase-producing bacterial populations on catfish tissue. Results obtained in this study suggest that extracellular DNase-producing bacteria play a large role in catfish spoilage and support the need for further research on the role of Aeromonas spp. in fish spoilage. Rahnella spp. was isolated from catfish fillets in this study and identified, for the first time, as DNase producing bacteria.
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Hickey, M.E., Besong, S.A., Kalavacharla, V. et al. Identification of extracellular DNase-producing bacterial populations on catfish fillets during refrigerated storage. Food Sci Biotechnol 22, 87–92 (2013). https://doi.org/10.1007/s10068-013-0012-1
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DOI: https://doi.org/10.1007/s10068-013-0012-1