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Phytochemical profiles and antioxidant activities of Serbian table and wine grapes

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Abstract

Three table grapes and 4 wine grapes collected from a southern Serbian vineyard were evaluated and compared for their antioxidant properties and phenolic profile. Among the varieties tested, ‘Cabernet Sauvignon’ contained the highest total phenolic content with 173.6 mg/100 g of fresh weight. Also, the total flavonoid and antocyanin content of ‘Cabernet Sauvignon’ was significantly higher from the other. ‘Cabernet Sauvignon’ and ‘Merlot’ had the strongest DPPH· radicals scavenging activity (1,318.6 and 1,282.0 μmol Trolox equivalent/100 g, respectively). ‘Cabernet Sauvignon’, ‘Merlot’, ‘Prokupac’, ‘Vranac’, ‘Muscat Hamburg’, and ‘Ribier’ grape varieties were found to be rich in malvidin-3-O-glucoside, while ‘Cardinal’ grape variety was found to be rich in peonidin-3-O-glucoside. The following compounds were also identified and quantified using HPLC-DAD: 2 flavan-3-ols and 4 hydroxycinnamic acids in all grape samples. The results suggested that phytochemicals in the selected table and wine grapes have potent antioxidant activities in correlation with phenolic content.

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References

  1. Blum M. Designing food for better health. Int. Food Ingredients 3: 25–29 (1996)

    Google Scholar 

  2. Pinheiro ES, Correia da Costa JM, Clemente E. Total phenolics and total anthocyanins found in grape from Benitaka cultivar (Vitis vinifera L.). J. Food Technol. 7: 78–83 (2009)

    CAS  Google Scholar 

  3. Yang J, Martinson TE, Liu RH. Phytochemical profiles and antioxidant activities of wine grapes. Food Chem. 116: 332–339 (2009)

    Article  CAS  Google Scholar 

  4. Mané C, Souquet JM, Ollé D, Verriés C, Véran F, Mazerolles G, Cheynier V, Fulcrand H. Optimization of simultaneous flavanol, phenilic acid, and anthocyanin extraction from grapes using an experimental design: Application to the characterization of ‘Champagne’ grape varieties. J. Agr. Food Chem. 55: 7224–7233 (2007)

    Article  Google Scholar 

  5. Arozarena I, Ayestaran B, Cantalejo MJ, Navarro M, Vera M, Abril I, Casp A. Anthocyanin composition of ‘Tempranillo’, ‘Garnacha’, and ‘Cabernet Sauvignon’ grapes from high- and low-quality vineyards over two years. Eur. Food Res. Technol. 214: 303–309 (2002)

    Article  CAS  Google Scholar 

  6. Singleton VL, Timberlake CF, Lea AGH. The phenolic cinnamates of white grapes and wine. Method. Enzymol. 299: 152–178 (1999)

    Article  CAS  Google Scholar 

  7. Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555–559 (1999)

    Article  Google Scholar 

  8. Yang J, Meyers KJ, van der Heide J, Liu RH. Varietal differences in phenolic content and antioxidant and antiproliferate activities of onions. J. Agr. Food Chem. 52: 6787–6793 (2004)

    Article  CAS  Google Scholar 

  9. Boyes MJ, Wrolstad RE. Anthocyanin composition of red raspberry juice: Influences of cultivar, processing, and environmental factors. J. Food Sci. 58: 1135–1141 (1993)

    Article  Google Scholar 

  10. Lachman J, Sulc M, Schilla M. Comparison of the total antioxidant status of Bohemian wines during the wine-making process. Food Chem. 103: 802–807 (2007)

    Article  CAS  Google Scholar 

  11. Turkoglu A, Duru M, Mecan N, Kivrak I, Gezer K. Antioxidant and antimicrobial activities od Laetiporus sulphurous (Bull) Murrill. Food Chem. 101: 267–273 (2007)

    Article  CAS  Google Scholar 

  12. Zhou K, Hao J, Griffey C, Chung H, O’Keefe SF, Chen J, Hogan S. Antioxidant properties of Fusarium head blight-resistant andsusceptible soft red winter wheat grains grown in Virginia. J. Agr. Food Chem. 55: 3729–3736 (2007)

    Article  CAS  Google Scholar 

  13. Lamuela-Raventos RM, Waterhouse AL. A direct HPLC separation of wine phenols. Am. J. Enol. Viticult. 45: 1–5 (1994)

    CAS  Google Scholar 

  14. Gomes-Alonso S, Garcia-Romero E, Hermosin-Gutierrez I. HPLC analysis of diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescence. J. Food Compos. Anal. 20: 618–626 (2007)

    Article  Google Scholar 

  15. Milis M, Ljubenkov I, Piskernik S, Pezo I, Terpinc P, Boban M. Polyphenolic profile, antioxidant properties, and antimicrobial activity of grape skin extracts of 14 Vitis vinifera grown in Dalmatia (Croatia). Food Chem. 119: 715–723 (2010)

    Article  Google Scholar 

  16. Perez-Magarino S, Gonzales-San Jose MJ. Polyphenols and color variability of red wines made from grape harvested at different ripeness grade. Food Chem. 96: 197–208 (2006)

    Article  CAS  Google Scholar 

  17. Cantos E, Espin JC, Tomas-Barberan FA. Varietal differences among the polyphenol profile of seven table grape cultivars studied by LC-DAD-MS-MS. J. Agr. Food Chem. 50: 5691–5696 (2002)

    Article  CAS  Google Scholar 

  18. Guerrero RF, Liazid A, Palma M, Puertas B, Gonzalez-Barrio R, Gil-Izquierdo A, Garcia-Barroso C, Cantos-Villar E. Phenolic characterization of red grape autochthonous to Andalusia. Food Chem. 112: 949–955 (2009)

    Article  CAS  Google Scholar 

  19. Hogan S, Zhang I, Janrong L, Zoecklein B, Zhou K. Antioxidant properties and bioactive components of ‘Norton’ (Vitis aestivalis) and ‘Cabernet Franc’ (Vitis vinifera) wine grapes. LWT-Food Sci. Technol. 42: 1269–1274 (2009)

    Article  CAS  Google Scholar 

  20. Yi C, Shi J, Kramer J, Xue S, Jiang Y, Zhang M, Ma Y, Pohorly J. Fatty acid composition and phenolic antioxidants of winemaking pomace power. Food Chem. 114: 570–576 (2009)

    Article  CAS  Google Scholar 

  21. Postrana-Bonilla E, Akon CC, Sellappan S, Krewer E. Phenol content and antioxidant capacity of ‘Muscadine’ grapes. J. Agr. Food Chem. 51: 5497–5503 (2003)

    Article  Google Scholar 

  22. Mattivi F, Zulian C, Valenti L. Wine biodiversity, technology, and antioxidants. Ann. NY Acad. Sci. 957: 27–57 (2002)

    Article  Google Scholar 

  23. Palma M, Pineiro Z, Barroso CG. Stability of phenolic compounds during extraction with superheater solvents. J. Chromatogr. A 921: 169–174 (2001)

    Article  CAS  Google Scholar 

  24. Dias FS, Lovillo MP, Barroso CG, David JM. Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence, Microchem. J. 96: 17–20 (2010)

    Article  CAS  Google Scholar 

  25. Katalinic V, Mozina SS, Sroza D, Generalic I, Abramovic H, Milos M, Ljubenkov I, Piskernic S, Pezo I, Terpinc P, Boban M. Polyphenolic profile, antioxidant properties, and antimicrobial activity of grape skin extracts of 14 Vitis vinifera varieties grown in Dalmatia (Croatia). Food Chem. 119: 715–723 (2010)

    Article  CAS  Google Scholar 

  26. Ivanova V, Stefova M, Chinnici F. Determination of the polyphenol contents in Macedonian grapes and wines by standardized spectrophotometric methods. J. Serb. Chem. Soc. 75: 45–59 (2010)

    Article  CAS  Google Scholar 

  27. Kallithraka S, Mohdaly AA, Makris DP, Kefalus P. Determination of major anthocyanin pigments in Hellenic native grape varieties (Vitis vinifera sp.): Association with antiradical activity. J. Food Compos. Anal. 18: 375–386 (2005)

    Article  CAS  Google Scholar 

  28. Kallithraka S, Aliaj L, Makris DP, Kefalus P. Anthocyanin profiles of major red grape (Vitis vinifera L.) varieties cultivated in Greece and their relationship with in vitro antioxidant characteristics Int. J. Food Sci. Tech. 44: 2385–2393 (2009)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Sciences and Mathematics, Department of Chemistry, University of Niš, Višegradska 33, P.O. Box 224, 18000, Niš, Serbia

    Milan N. Mitić, Mirjana V. Obradović & Snežana S. Mitić

  2. UMR 1083 Sciences Pour l’oenologie France, INRA, 2 Place Viala, F-34060, Montpellier, France

    Jean-Marc Souquet

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  1. Milan N. Mitić
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  2. Jean-Marc Souquet
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  3. Mirjana V. Obradović
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  4. Snežana S. Mitić
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Correspondence to Milan N. Mitić.

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Mitić, M.N., Souquet, JM., Obradović, M.V. et al. Phytochemical profiles and antioxidant activities of Serbian table and wine grapes. Food Sci Biotechnol 21, 1619–1626 (2012). https://doi.org/10.1007/s10068-012-0215-x

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  • DOI: https://doi.org/10.1007/s10068-012-0215-x

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