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Development and application of a rapid and simple loop-mediated isothermal amplification method for food-borne Salmonella detection

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Abstract

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65°C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.

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Authors and Affiliations

  1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China

    Xihong Zhao, Jin Chu, Zhenbo Xu, Lin Li, Xiaowei He & Liansheng Yang

  2. Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, 430073, Hubei China

    Xihong Zhao

  3. Food Safety Key Laboratory of Guangdong Povince, College of Food Science, South China Agricultural University, Guangzhou, China

    Li Wang

  4. Guangzhou Medical College, Guangzhou, China

    Yanmei Li

  5. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Yanyan Li

  6. Department of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD, 21201, USA

    Zhenbo Xu & Mark E. Shirtliff

  7. Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD, USA

    Mark E. Shirtliff

  8. Zhongshan Supervision Testing Institute of Quality & Metrology, Zhongshan, China

    Yao Liu

  9. Guangzhou Wondfo Biotechnology Company, Guangzhou, China

    Jihua Wang

Authors
  1. Xihong Zhao
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  2. Li Wang
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  3. Jin Chu
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  4. Yanmei Li
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  5. Yanyan Li
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  6. Zhenbo Xu
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  7. Lin Li
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  8. Mark E. Shirtliff
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  9. Xiaowei He
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  10. Yao Liu
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  11. Jihua Wang
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  12. Liansheng Yang
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Corresponding author

Correspondence to Zhenbo Xu.

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Zhao, X., Wang, L., Chu, J. et al. Development and application of a rapid and simple loop-mediated isothermal amplification method for food-borne Salmonella detection. Food Sci Biotechnol 19, 1655–1659 (2010). https://doi.org/10.1007/s10068-010-0234-4

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  • DOI: https://doi.org/10.1007/s10068-010-0234-4

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