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Elevated plasma levels of specific antiplatelet glycoprotein autoantibodies in patients with primary Sjögren syndrome with thrombocytopenia

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Abstract

Introduction

Thrombocytopenia is one of the primary Sjögren’s syndrome (pSS) hematological manifestations. The objective of this study was to evaluate the possible roles of antiplatelet glycoprotein autoantibodies in the pathogenesis of thrombocytopenia in primary Sjögren’s syndrome (pSS).

Methods

The level of plasma anti-glycoprotein Ib, IIIa and IIb/IIIa autoantibodies in 36 pSS patients without thrombocytopenia and 35 pSS patients with thrombocytopenia, 36 Idiopathic thrombocytopenic purpura (ITP) patients and 39 normal control were measured with enzyme-linked immunosorbent assay (ELISA).

Results

The level of anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies (A490) in the pSS with thrombocytopenia was significantly higher than that of pSS without thrombocytopenia (0.813 ± 0.161 vs 0.688 ± 0.133; 0.917 ± 0.094 vs 0.802 ± 0.070; 0.911 ± 0.125 vs 0.782 ± 0.109). Incidences of the anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies in the pSS with thrombocytopenia was significantly higher than that of pSS without thrombocytopenia (25.7% vs 0%; 65.7% vs 11.1%; 31.4% vs 0%). In patients with pSS, there was a lower platelet count in anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies positive patients ((25.67 ± 5.5) × 10^9/L vs (116.8 ± 84.52) × 10^9/L; 29.04 ± 11.33 × 10^9/L vs (152.0 ± 75.47) × 10^9/L; (31.55 ± 14.0) × 10^9/L vs (118.8 ± 85.24) × 10^9/L).

Conclusion

Elevated plasma levels of anti-platelet glycoprotein autoantibodies may play a role in the pathogenesis of thrombocytopenia in pSS.

Key Points

• The level of anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies (A490) in the pSS with thrombocytopenia was increased.

• Incidences of the anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies in the pSS with thrombocytopenia was increased.

• In patients with pSS, there was a lower platelet count in anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies positive patients.

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Funding

National Nature Science Foundation of China (82170217);

Key Project of Tianjin Natural Science Foundation, (grant number: 20JCZDJC00410),;

CAMS Innovation Fund for Medical Sciences (CIFMS) (grant numbers: 2021-I2M-1-073, 2022-I2M-C&T-B-091),;

Clinical research project of Tianjin Society of Hematology and Regenerative Medicine (No. 2022 TSHRM08003).

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Author notes

  1. Yingxin Ruan and Hongchen Bi contribute equally to this work.

Authors and Affiliations

  1. Department of Nephrology, General Hospital of Tianjin Medical University, Tianjin, China

    Yingxin Ruan

  2. School of Medical Laboratory, Tianjin Medical University, Tianjin, China

    Hongchen Bi, Xiaoli Luo & Yujie Cui

  3. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Hematopoietic Stem Cell Transplantation Center, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China

    Aiming Pang

  4. Department of Blood Transfusion, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China

    Pengyu Zhang

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  1. Yingxin Ruan
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  2. Hongchen Bi
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  3. Xiaoli Luo
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  4. Aiming Pang
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  5. Pengyu Zhang
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  6. Yujie Cui
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Correspondence to Pengyu Zhang or Yujie Cui.

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Ruan, Y., Bi, H., Luo, X. et al. Elevated plasma levels of specific antiplatelet glycoprotein autoantibodies in patients with primary Sjögren syndrome with thrombocytopenia. Clin Rheumatol 43, 307–314 (2024). https://doi.org/10.1007/s10067-023-06818-y

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