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Impact of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid

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Abstract

Of the glucose degradation products (GDPs) in glucose-rich peritoneal dialysate, we investigated the influence of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on the cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid (L-H PDF) using human peritoneal mesothelial cells (HPMC). We prepared acidified filtration-sterilized PDF (glucose concentration 3.86%) containing eight types of added GDP [3,4-DGE, glyoxal (GO), methylglyoxal (MGO), 3-deoxyglucosone (3-DG), formaldehyde (FA), acetaldehyde (AA), 5-hydroxymethyl-2-furaldehyde (5-HMF), and furfural (FF)] or seven types of GDP (GO, MGO, 3-DG, FA, AA, 5-HMF, and FF). HPMC were exposed to these two types of solution and acidic heat-sterilized PDF (glucose concentration 3.86%, L-H 3.86) for 4 h. Cell viability was determined by 3,(4,5-dimethythiazol-2-yl)2,5-diphenyl-terazolium bromide (MTT) assay. MTT viability was decreased significantly compared with the control when treated with L-H 3.86 or acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing eight GDPs. However, no significant decrease in MTT viability was observed when HPMC were treated with acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing seven GDPs. Thus, 3,4-DGE strongly affects the cytotoxicity of L-H PDF. It is suggested that the cytotoxicity of L-H PDF is based on the presence of 3,4-DGE.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Faculty of Medicine, Oita University, Yufu, Oita, 879-5593, Japan

    Tadashi Tomo MD, Eiji Okabe MD, Tomohiko Iwashita MD, Kazuhiro Matsuyama MD & Jun-ichi Kadota MD

  2. JMS Co., Ltd., Hiroshima, Japan

    Takashi Yamamoto MD & Shinji Namoto MD

Authors
  1. Tadashi Tomo MD
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  2. Eiji Okabe MD
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  3. Takashi Yamamoto MD
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  4. Shinji Namoto MD
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  5. Tomohiko Iwashita MD
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  6. Kazuhiro Matsuyama MD
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  7. Jun-ichi Kadota MD
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Correspondence to Tadashi Tomo MD.

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Tomo, T., Okabe, E., Yamamoto, T. et al. Impact of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid. J Artif Organs 10, 47–51 (2007). https://doi.org/10.1007/s10047-006-0358-8

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