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A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer

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Abstract

This paper describes a method for measuring the three-dimensional (3D) refractive-index distribution in a single cell. The method can be used to observe the distribution of cell components without fluorescence staining. The two-dimensional optical path length distributions from multiple directions are obtained by non-contact rotation of the cell. These optical path lengths are converted into the line integrals of the refractive index, and the 3D refractive-index distribution is reconstructed by means of computed tomography. The refractive-index distribution in a breast cancer cell can be measured using a phase-shifting Mach—Zehnder interferometer in conjunction with proximal two-beam optical tweezers.

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Authors and Affiliations

  1. Faculty of Engineering, Kagawa University, 2217-20, Hayashicho, Takamatsu, 761-0396, Japan

    Toshiki Yasokawa, Ichirou Ishimaru & Masahiro Kondo

  2. Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan

    Shigeki Kuriyama & Tsutomu Masaki

  3. Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Kaoru Takegawa & Naotaka Tanaka

Authors
  1. Toshiki Yasokawa
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  2. Ichirou Ishimaru
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  3. Masahiro Kondo
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  4. Shigeki Kuriyama
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  5. Tsutomu Masaki
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  6. Kaoru Takegawa
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  7. Naotaka Tanaka
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Yasokawa, T., Ishimaru, I., Kondo, M. et al. A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer. OPT REV 14, 161–164 (2007). https://doi.org/10.1007/s10043-007-0161-7

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  • DOI: https://doi.org/10.1007/s10043-007-0161-7

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