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The expression of neuroglobin in astrocytoma

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Abstract

Neuroglobin (NGB) is a characterized heme globin that is widely expressed in the vertebrate central and peripheral nervous system as well as retina and endocrine tissues. However, to date, it is not determined whether functional NGB is expressed in cells of glia origin. In this study, we aimed to explore the detailed expression of NGB in a rat astrocytoma cell line (C6) and human astrocytoma cell line (U251) by reverse transcription-polymerase chain reaction, immunofluorescence, and Western blotting, and to detect the expression of NGB in human astrocytoma tissues by an immunohistochemical method. We found that NGB was present in a rat astrocytoma cell line (C6), human astrocytoma cell line (U251), and human astrocytoma tissues. The expression and potential roles of NGB in astrocytomas may provide insight into the mechanisms of tumor cells to adapt and survive in hypoxic microenvironments and also represent a novel therapeutic approach to astrocytomas.

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Acknowledgments

The study was supported by National Natural Science Foundation of China (NSFC, No. 30470660) and the Natural Science Foundation of Sichuan Province (No. 05JY029-020, No. 2011JY0007). We thank Prof. Wentong Meng and Dr. Guohua Huang for their excellent technical assistance, and we sincerely thank the editors and anonymous reviewers for their constructive comments in revising this manuscript.

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Authors and Affiliations

  1. Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, China

    Haojie Qin, Changquan Zhang, Lin Zhang, Mingyuan Li & Peng Guan

  2. School of Forensic Medicine, Henan University of Science and Technology, 471003, Luoyang, China

    Haojie Qin

  3. Department of Forensic Science, School of Basic Medical Sciences, Central South University, 410013, Changsha, Hunan, China

    Yadong Guo

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  1. Haojie Qin
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  2. Yadong Guo
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  3. Changquan Zhang
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  4. Lin Zhang
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  5. Mingyuan Li
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  6. Peng Guan
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Corresponding author

Correspondence to Peng Guan.

Additional information

H. Qin and Y. Guo contributed equally to this work.

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Qin, H., Guo, Y., Zhang, C. et al. The expression of neuroglobin in astrocytoma. Brain Tumor Pathol 29, 10–16 (2012). https://doi.org/10.1007/s10014-011-0066-9

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