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Development of a mimetic system for electrochemical detection of glutamate

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Abstract

This paper describes the development of a biosensor to detect neurodegenerative diseases, focusing on Alzheimer’s disease, the most common type of dementia, based on the use of a small protein-like chain designed to mimic a peptide that recognizes glutamate, the main excitatory neurotransmitter present in the central nervous system of mammals. This system is based on the immobilization of the mimetic peptide for glutamate onto graphite electrodes. The produced bioelectrode showed interesting characteristics, such as short response time (about 10 s) and linear response range between 1 and 10 mmol L−1 for glutamate, indicating a promising approach for the diagnosis of neurological diseases. In addition, it was possible to observe differences in charge transfer resistance and in surface topography of the electrode, after the interaction with the glutamate target. Theoretical calculations suggest that the anchoring of glutamate indicates conformational changes in the peptide. The mimetic bioelectrode discriminates samples from patients with Alzheimer’s disease.

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Acknowledgments

The authors are grateful for the financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Also, we would like to thank teacher Abílio Borghi for the review of the English manuscript.

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Authors and Affiliations

  1. Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Brazil

    Lívia M. Alves, Ana C. H. Castro, Luiz C. O. Júnior, Luiz R. Goulart, Ana M. Bonetti, Carlos Ueira-Vieira & Ana G. Brito-Madurro

  2. Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Brazil

    Samuel M. Oliveira, Antonio E. H. Machado & João M. Madurro

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  1. Lívia M. Alves
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  2. Ana C. H. Castro
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  3. Samuel M. Oliveira
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  4. Luiz C. O. Júnior
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  5. Luiz R. Goulart
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  6. Ana M. Bonetti
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  7. Antonio E. H. Machado
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  8. Carlos Ueira-Vieira
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  9. João M. Madurro
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  10. Ana G. Brito-Madurro
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Correspondence to Ana G. Brito-Madurro.

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Alves, L.M., Castro, A.C.H., Oliveira, S.M. et al. Development of a mimetic system for electrochemical detection of glutamate. J Solid State Electrochem 20, 2479–2489 (2016). https://doi.org/10.1007/s10008-016-3236-1

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  • DOI: https://doi.org/10.1007/s10008-016-3236-1

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