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Introducing curcumin as an electrochemical DNA hybridization indicator and its application for detection of human interleukin-2 gene

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Abstract

In this paper, the application of curcumin (CU) as a non-toxic electrochemical DNA hybridization indicator was described. Hybridization investigations on a pencil graphite electrode surface as a transducer using oligonucleotides containing only one base type, including poly A, poly T, poly C, and poly G as probe and as related complementary/non-complementary sequences, showed that CU has no specific interaction with each of the oligonucleotides of DNA. Furthermore, results showed good interaction between CU and the hybridized form of oligonucleotides; thus, the extent of hybridization was evaluated based on the difference between differential pulse voltammetry (DPV) signals of CU accumulated on the probe-pencil graphite electrode (PGE) and CU accumulated on the probe-target-PGE. Then, the developed biosensor was successfully applied for the detection of short sequences of human interleukin-2 (hIL-2) gene as a model. A hybridization experiment with non-complementary oligonucleotide showed that the suggested DNA sensor responds selectively to the target. At optimized conditions, two linear ranges were obtained for hIL-2 gene, first from 50 to 1000 pM and second from 0.01 to 1 μM with a detection limit of 12 pM. 7.0) containing 20 mM NaCl.

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Acknowledgments

This work was financially supported by the Iran National Science Foundation, INSF (research proposal no.: 92020049), and University of Tabriz.

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Authors and Affiliations

  1. Electroanalytical Chemistry Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

    Esmaeel Alipour & Habibeh Shahabi

  2. Electroanalytical Chemistry Laboratory, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

    Tohid Mahmoudi-Badiki

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  1. Esmaeel Alipour
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  2. Habibeh Shahabi
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Correspondence to Esmaeel Alipour.

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Alipour, E., Shahabi, H. & Mahmoudi-Badiki, T. Introducing curcumin as an electrochemical DNA hybridization indicator and its application for detection of human interleukin-2 gene. J Solid State Electrochem 20, 1645–1653 (2016). https://doi.org/10.1007/s10008-016-3168-9

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  • DOI: https://doi.org/10.1007/s10008-016-3168-9

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