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A hydrogen peroxide biosensor with high stability based on gelatin-multiwalled carbon nanotubes modified glassy carbon electrode

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Abstract

A biosensor with high stability was prepared to determine hydrogen peroxide (H2O2). This hydrogen peroxide biosensor was obtained by modifying glassy carbon electrode (GCE) with a composite film composed of gelatin-multiwalled carbon nanotubes. Catalase (Cat) was covalently immobilized into gelatin-multiwalled carbon nanotubes modified GCE through the well-known glutaraldehyde (GAD) chemistry in order to enhance the stability of electrodes. The enzyme sensor can achieve direct electrochemical response of hydrogen peroxide. The cyclic voltammograms at different scan rates, electrochemical impedance spectroscopy (EIS), and scanning electron microscope (SEM) tests indicate that the enzyme sensor performs positively on increasing permeability, reducing the electron transfer resistance, and improving the electrode performance. The linear response of standard curve for H2O2 is in the range of 0.2 to 5.0 mM with a correlation coefficient of 0.9972, and the detection limit of 0.001 mM. A high operational and storage stability is demonstrated for the biosensor. The peak potential at room temperature in two consecutive weeks stays almost consistent, and the enzyme activity is kept stable even after 30 days in further study.

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Authors and Affiliations

  1. State Key Laboratory of Chemical Resource Engineering, The Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing, 100029, People’s Republic of China

    Yulin Wang, Weijie Zhang & Yaqin Huang

  2. Key Lab of Organic Optoelectronics & Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing, 100084, People’s Republic of China

    Tianyu Li

Authors
  1. Yulin Wang
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  2. Tianyu Li
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  3. Weijie Zhang
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  4. Yaqin Huang
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Correspondence to Yaqin Huang.

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Wang, Y., Li, T., Zhang, W. et al. A hydrogen peroxide biosensor with high stability based on gelatin-multiwalled carbon nanotubes modified glassy carbon electrode. J Solid State Electrochem 18, 1981–1987 (2014). https://doi.org/10.1007/s10008-014-2441-z

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  • DOI: https://doi.org/10.1007/s10008-014-2441-z

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