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Evaluation of matrix metalloproteinase-1, -2, -3, -7, and -13 gene polymorphisms in patients with chronic periodontitis and healthy controls

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Abstract

Objectives

The current study aimed to investigate the association of matrix metalloproteinase- (MMP-) 1, -2, -3, -7, and -13 gene polymorphisms with chronic periodontitis (CP) in an Iranian population.

Materials and Methods

In this case–control study, 87 subjects with CP and 89 periodontally healthy subjects were allocated to case and control groups, respectively. Subjects’ venous blood samples (5 cc) were collected, and DNA extraction was performed. A spectrophotometer was utilized to assess the concentration of extracted DNAs. The desired gene polymorphisms were examined using restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) followed by electrophoresis. Statistical analyses were done using the Pearson Chi-Square test, odds ratio, and t-Test using SPSS Version 28.

Results

The MMP-1 (-1607 1G/2G) rs1799750, MMP-3 (-1171 5A/6A) rs3025058, and MMP-7 (-181 A/G) rs11568818 gene polymorphisms significantly differed between case and control groups (PV = 0.019, 0.007, and 0.028, respectively). In contrast, the gene polymorphisms of MMP-2 (-1306 C/T) rs243865 and MMP-13 (-77 A/G) rs2252070 did not make a significant difference. Regarding allele frequencies, the presence of the 2G allele in the MMP-1 (-1607) rs1799750 genotype increased the CP susceptibility significantly, while subjects with the 6A allele in their MMP-3 (-1171) rs3025058 genotype showed significantly lower susceptibility to CP (PV = 0.008 and < 0.001, respectively).

Conclusion

In the studied population, gene polymorphisms in the DNA sequences of MMP-1 (-1607 1G/2G) rs1799750, MMP-3 (-1171 5A/6A) rs3025058, and MMP-7 (-181 A/G) rs11568818 may have impacts on CP incidence.

Clinical Relevance

Clinicians should be cautious about the association between MMP-1, MMP-3, and MMP-7 gene polymorphisms and the incidence of chronic periodontitis during periodontal treatment planning.

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Data availability

The data that support the findings of this study are not openly available due to reasons of sensitivity and are available from the corresponding author upon reasonable request.

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Funding

No funding was obtained for this study.

Author information

Authors and Affiliations

  1. Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Leila Saremi & Mahdi Kadkhodazadeh

  2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Leila Saremi

  3. Division of Oral and Maxillofacial Surgery, School of Dentistry, University of California, Los Angeles, Los Angeles, CA, USA

    Saharnaz Esmaeili

  4. Dentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Saharnaz Esmaeili & Soheil Shahbazi

  5. Department of Epidemiology and Biostatistics, Faculty of Health, Qom University of Medical Sciences, Qom, Iran

    Mohammad Ebrahim Ghaffari

  6. Department of Biology Education, Farhangian University, Tehran, Iran

    Shirin Lotfipanah

Authors
  1. Leila Saremi
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  2. Saharnaz Esmaeili
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  3. Mohammad Ebrahim Ghaffari
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  4. Soheil Shahbazi
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  6. Mahdi Kadkhodazadeh
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Contributions

Leila Saremi: Conceptualization, Methodology, Project administration, Data curation.

Saharnaz Esmaeili: Data curation, Writing – original draft.

Mohammad Ebrahim Ghaffari: Formal analysis.

Soheil Shahbazi: Investigation, Writing – original draft.

Shirin Lotfipanah: Data curation.

Mahdi Kadkhodazadeh: Conceptualization, Methodology, Supervision, Resources, Writing – review & editing.

Corresponding author

Correspondence to Mahdi Kadkhodazadeh.

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Competing interests

The authors declare no competing interests.

Ethics approval and consent to participate

Following the local ethics committee approval (DRC.REC.1399.099), written consent forms were signed by the patients.

Conflict of interest

The authors declare that they have no conflict of interest.

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Saremi, L., Esmaeili, S., Ghaffari, M.E. et al. Evaluation of matrix metalloproteinase-1, -2, -3, -7, and -13 gene polymorphisms in patients with chronic periodontitis and healthy controls. Clin Oral Invest 27, 7417–7423 (2023). https://doi.org/10.1007/s00784-023-05331-5

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  • DOI: https://doi.org/10.1007/s00784-023-05331-5

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