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Characterising salivary peptidome across diurnal dynamics and variations induced by sampling procedures

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

This study aimed to characterise diurnal dynamics of salivary peptidome and variations induced by sampling procedures.

Materials and methods

A supervised short-term longitudinal study was conducted amongst ten healthy participants. Saliva samples were collected by different procedures (stimulated/unstimulated conditions, forepart/midstream segments) on three consecutive days. The peptidome compositions of saliva samples were analysed using matrix-assisted laser desorption/ionisation-time of flight-mass spectrometry (MALDI-TOF MS).

Results

The salivary peptidome exhibited a stable trend generally, even though some diurnal dynamics happened in aspects of both overall structure and certain single peptides. The results indicated saliva samples collected under unstimulated and stimulated conditions have significantly different structures of peptidome, whilst the peptidome profile of stimulated saliva was more abundant than that of unstimulated saliva. It was also indicated that the midstream segment effect might exist in the segmented process of saliva sampling.

Conclusions

In summary, salivary peptidome was able to maintain stability though some dynamic changes might happen within a short-term period. Stimulated and unstimulated saliva samples had significantly different peptidome profiles, whilst the stimulated whole saliva was a larger pool of low molecular weight peptides.

Clinical relevance

The stability of the salivary peptidome highlights the reliability of salivary peptidome as a source of diagnostic biomarker. We recommend keeping one collection condition (stimulated/unstimulated) consistently within one study on salivary peptidome. Stimulated whole saliva would be preferred if more abundant peptidome profile is needed.

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Data availability

Data sets used for analyses in the present study are available upon request.

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Funding

The work was supported by the National Natural Science Foundation of China (grant number: 81801037), the Program for New Clinical Techniques and Therapies of Peking University School and Hospital of Stomatology (grant number: PKUSSNCT-21B15), the Capital’s Funds for Health Improvement and Research (grant number: CFH2022-2G-4105), and the Ministry of Science and Technology of the People’s Republic of China (grant number: 2018FY101005).

Author information

Author notes

  1. Ce Zhu and Chao Yuan contributed equally to this study and share the first authorship.

Authors and Affiliations

  1. Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, 100081, People’s Republic of China

    Ce Zhu, Chao Yuan, Fangqiao Wei, Xiangyu Sun & Shuguo Zheng

  2. Department of Preventive Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Dentistry, Shanghai, 200011, People’s Republic of China

    Ce Zhu

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Contributions

All authors have made substantial contributions to the conception and design of the study. All authors have been involved in data collection, and Z.C. has been involved in data analysis. All authors have been involved in data interpretation, and Z.C. has been involved in drafting of the manuscript. X.Y.S. and S.G.Z have been involved in revising it critically, and all authors have given the final approval of the version to be published.

Corresponding authors

Correspondence to Xiangyu Sun or Shuguo Zheng.

Ethics declarations

Ethics approval

This study was approved by the Peking University School and Hospital of Stomatology Ethics Committee (PKUSSIRB-201944061 and PKUSSIRB-202274053). All the procedures were performed in accordance with the Declaration of Helsinki and the STROBE guidelines.

Consent to participate

Informed consent was obtained from all participants before the study began.

Conflict of interest

The authors declare no competing interests.

The following four comparisons: UWS.F vs UWS.M, MSWS.F vs MSWS.M, UWS.F vs MSWS.F, UWS.M vs MSWS.M were reported. UWS.F, the forepart segment of unstimulated whole saliva. UWS.M, the midstream segment of unstimulated whole saliva. MSWS.F, the forepart segment of mechanically stimulated whole saliva. MSWS.M, the midstream segment of mechanically stimulated whole saliva (similarly hereinafter).

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Zhu, C., Yuan, C., Wei, F. et al. Characterising salivary peptidome across diurnal dynamics and variations induced by sampling procedures. Clin Oral Invest 27, 285–298 (2023). https://doi.org/10.1007/s00784-022-04722-4

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  • DOI: https://doi.org/10.1007/s00784-022-04722-4

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