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A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

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Clinical Oral Investigations Aims and scope Submit manuscript

An Erratum to this article was published on 02 March 2014

Abstract

Objectives

The aim of this study was to investigate the capability of a novel reference point indentation apparatus to test the indentation properties of root canal surface dentine treated with three intracanal medicaments used in endodontic regeneration.

Materials and methods

Immature human premolars were selected (n = 22). Four specimens were obtained from each root and randomly assigned to three treatment groups and a control group. Each specimen was exposed to one of the three treatment pastes (triple antibiotic (TAP), double antibiotic (DAP), or calcium hydroxide (Ca(OH)2)) or neutral deionized water (control) for 1 or 4 weeks. After each time interval, the indentation properties of the root canal dentine surfaces were measured using a BioDent reference point indenter. Two-way ANOVA and Fisher's protected least significant differences were used for statistical analyses.

Results

Significant differences in indentation parameters and estimated hardness between all groups at both time points were found. TAP-treated dentine had the highest significant indentation parameters, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Furthermore, TAP-treated dentine had the lowest significant estimated hardness, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively.

Conclusion

BioDent reference point indenter was able to detect significant differences in indentation properties of root canal dentine treated with various medicaments.

Clinical relevance

The use of a reference point indenter is a promising approach to characterize the indentation properties of root canal surfaces without any surface modification. This might provide an in vitro mechanical measurement that is more representative of the actual clinical situation.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, 1121W. Michigan St., Indianapolis, IN, 46202, USA

    Ghaeth H. Yassen, Tien-Min G. Chu & Jeffrey A. Platt

  2. Department of Pediatric, Orthodontic and Preventive Dentistry, Mosul University School of Dentistry, Mosul, Iraq

    Ghaeth H. Yassen

  3. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA

    Maxime A. Gallant & Matthew R. Allen

  4. Department of Endodontics, Indiana University School of Dentistry, Indianapolis, IN, USA

    Mychel M. Vail

  5. Department of Endodontics, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA

    Peter E. Murray

Authors
  1. Ghaeth H. Yassen
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  2. Tien-Min G. Chu
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  3. Maxime A. Gallant
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  4. Matthew R. Allen
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  5. Mychel M. Vail
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  6. Peter E. Murray
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  7. Jeffrey A. Platt
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Correspondence to Ghaeth H. Yassen.

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Yassen, G.H., Chu, TM.G., Gallant, M.A. et al. A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation. Clin Oral Invest 18, 1569–1575 (2014). https://doi.org/10.1007/s00784-013-1125-x

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  • DOI: https://doi.org/10.1007/s00784-013-1125-x

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