Abstract
The aim of this in vitro study was to evaluate the relationship between laser fluorescence values and sealant penetration depth on occlusal fissures. One hundred and sixty-six permanent molars were selected and divided into four groups, which were each treated using a different sealant (two clear and two opaque). The teeth were independently measured twice by two experienced dentists using two laser fluorescence devices—DIAGNOdent (LF and LFpen)—before and after sealing, and then thermoclycled. After measuring, the teeth were histologically prepared and assessed for caries extension. Digital photographs of the cut sealed sites were assessed, and the sealant penetration depth was measured. All 166 sites were measured by one of the examiners taking as limits the outer and inner surface of the sealant into the fissure. For each device (LF and LFpen) and each group, the difference between the values at baseline and after sealing was plotted against the sealant penetration depth and scatter plots were provided. It could be observed that most of the points were concentrated around the zero line, for both LF and LFpen in the four groups. In conclusion, there is no relation between changes in DIAGNOdent values and increasing of depth sealant penetration within the occlusal fissures.
References
Manton DJ, Messer LB (1995) Pit and fissure sealants: another major cornerstone in preventive dentistry. Aust Dent J 40:20–29
Simonsen RJ (1991) Retention and effectiveness of dental sealant after 15 years. J Am Dent Assoc 122:34–42
Mertz-Fairhurst EJ, Curtis JW Jr, Ergle JW, Rueggeberg FA, Adair SM (1998) Ultraconservative and cariostatic sealed restorations: results at year 10. J Am Dent Assoc 129:55–66
Simonsen RJ (2002) Pit and fissure sealant: review of the literature. Pediatr Dent 24:393–414
Diniz MB, Rodrigues JA, Hug I, Cordeiro RC, Lussi A (2008) The influence of pit and fissure sealants on infrared fluorescence measurements. Caries Res 42:328–333
Hibst R, Paulus R, Lussi A (2001) A detection of occlusal caries by laser fluorescence: basic and clinical investigations. Med Laser Appl 16:295–213
Bader JD, Shugars DA (2004) A systematic review of the performance of a laser fluorescence device for detecting caries. J Am Dent Assoc 135:1413–1426
Rodrigues JA, Hug I, Diniz MB, Lussi A (2008) Performance of fluorescence methods, radiographic examination and ICDAS II on occlusal surfaces in vitro. Caries Res 42:297–304
Sheehy EC, Brailsford SR, Kidd EAM, Beighton D, Zoitopoulos L (2001) Comparison between visual examination and a laser fluorescence system for in vivo diagnosis of occlusal caries. Caries Res 35:421–426
Lussi A, Imwinkelried S, Pitts N, Longbottom C, Reich E (1999) Performance and reproducibility of a laser fluorescence system for detection of occlusal caries in vitro. Caries Res 33:261–266
Lussi A, Hellwig E (2006) Performance of a new laser fluorescence device for the detection of occlusal caries in vitro. J Dent 34:467–471
Diniz MB, Rodrigues JD, Paula AB, Cordeiro RD (2009) In vivo evaluation of laser fluorescence performance using different cut-off limits for occlusal caries detection. Lasers Med Sci 24:295–300
Lussi A, Reich E (2005) The influence of toothpastes and prophylaxis pastes on fluorescence measurements for caries detection in vitro. Eur J Oral Sci 113:141–144
Hosoya Y, Matsuzaka K, Inoue T, GW M (2004) Influence of tooth-polishing pastes and sealants on DIAGNOdent values. Quintessence Int 35:605–611
Anttonen V, Seppä L, Hausen H (2003) Clinical study of the use of the laser fluorescence device DIAGNOdent for detection of occlusal caries in children. Caries Res 37:17–23
Deery C, Iloya J, Nugent ZJ, Srinivasan V (2006) Effect of placing a clear sealant on the validity and reproducibility of occlusal caries detection by a laser fluorescence device: An in vitro study. Caries Res 40:186–193
Krause F, Braun A, Frentzen M, Jepsen S (2008) Effects of composite fissure sealants on IR laser fluorescence measurements. Lasers Med Sci 23:133–139
Takamori K, Hokari N, Okumura Y, Watanabe S (2001) Detection of occlusal caries under sealants by use of a laser fluorescence system. J Clin Laser Med Surg 19:267–271
Gostanian HV, Shey Z, Kasinathan C, Caceda J, Janal MN (2006) An in vitro evaluation of the effect of sealant characteristics on laser fluorescence for caries detection. Pediatr Dent 28:445–450
Celiberti P, Lussi A (2007) Penetration ability and microleakage of a fissure sealant applied on artificial and natural enamel fissure caries. J Dent 35:59–67
Francescut P, Zimmerli B, Lussi A (2006) Influence of different storage methods on laser fluorescence values: A two-year study. Caries Res 40:181–185
Deery C, Fyffe H, Nugent Z, Nuttall N, Pitts N (1995) The effect of placing a clear pit and fissure sealant on the validity and reproducibility of occlusal caries diagnosis. Caries Res 29:377–381
Carvalho JC, Ekstrand KR, Thylstrup A (1989) Dental plaque and caries on occlusal surfaces of first permanent molars in relation to stage of eruption. J Dent Res 68:773–779
Pearce EIF, Larsen M, Coote G (1999) Fluoride in enamel lining pits and fissures of the occlusal groove–fossa system in human molar teeth. Caries Res 33:196–205
Rock WP, Weatherill S, Anderson RJ (1990) Retention of three fissure sealants resins. The effects of etching agent and curing method. Results over 3 years. Br Dent J 168:232–325
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rodrigues, J.A., Diniz, M.B., Hug, I. et al. Relationship between DIAGNOdent values and sealant penetration depth on occlusal fissures. Clin Oral Invest 14, 707–711 (2010). https://doi.org/10.1007/s00784-009-0321-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00784-009-0321-1