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Vanadate as a new substrate for nucleoside phosphorylases

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Abstract

Orthovanadate was shown to serve as a substrate for nucleoside phosphorylases from Escherichia coli, Shewanella oneidensis, Geobacillus stearothermophilus, and Halomonas chromatireducens AGD 8-3. An exception is thymidine phosphorylase from the extremophilic haloalkaliphilic bacterium Halomonas chromatireducens AGD 8-3, which cannot catalyze the vanadolysis of nucleosides. The kinetic parameters of nucleoside vanadolysis were evaluated.

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Authors and Affiliations

  1. A.N. Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Science, Leninsky Prosp. 33, Build 2, Moscow, 119071, Russia

    Alexey N. Antipov, Natalya A. Okorokova, Tatyana N. Safonova & Vladimir P. Veiko

  2. Research Centre for Medical Genetics RAMS, Moscow, Russia

    Vladimir P. Veiko

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  1. Alexey N. Antipov
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  2. Natalya A. Okorokova
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  3. Tatyana N. Safonova
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  4. Vladimir P. Veiko
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Correspondence to Alexey N. Antipov.

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Antipov, A.N., Okorokova, N.A., Safonova, T.N. et al. Vanadate as a new substrate for nucleoside phosphorylases. J Biol Inorg Chem 27, 221–227 (2022). https://doi.org/10.1007/s00775-021-01923-2

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  • DOI: https://doi.org/10.1007/s00775-021-01923-2

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