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Resveratrol prevents ovariectomy-induced bone quality deterioration by improving the microarchitectural and biophysicochemical properties of bone

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Abstract

Introduction

Osteoporosis is a major health problem that is very common worldwide and is characterized by both low bone density and deterioration in bone quality. New treatment options without side effects have become an active area of research in recent years. This study was designed to investigate the preventive effects of resveratrol on bone quality deterioration caused by ovariectomy.

Materials and Methods

Sixty rats were randomly divided into five groups (12 animals per group): Control, Sham-operated (SHAM), ovariectomized (OVX), OVX + Resveratrol-40 mg/kg/day (OVX + Res40), OVX + Resveratrol-80 mg/kg/day (OVX + Res80). Resveratrol was administered by oral gavage (40 and 80 mg/kg/day) for ten weeks. Micro-CT measurements, biomechanical testing, Raman spectroscopy analysis, and RT-PCR analysis were performed. ALP, OCN, TAS, and TOS levels were also measured from blood serum.

Results

Bone strength, bone volume/total volume, trabecular volume, and trabecular thickness were higher in the OVX + RES-80 group than in the OVX group. Resveratrol increased osteogenic differentiation, as the expression of osteogenic markers ALP, Col1A1, Runx2, OPG, OCN increased in both OVX + RES-80 and OVX + RES-40 groups compared to the OVX group. 80 mg/kg/day resveratrol administration decreased the levels of ALP, OCN and TOS in ovariectomized rats. Raman spectroscopy findings showed a preventive effect of resveratrol administration against ovariectomy-induced deterioration in biophysiochemical properties of bone tissue.

Conclusion

This study revealed that administration of different doses of 80 mg/kg/day and 40 mg/kg/day of resveratrol had protective effects on bone quality deterioration caused by ovariectomy.

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Acknowledgements

The study was part of Sevval Ozturk's master's thesis.

Funding

This study was supported by the Research Fund of Pamukkale University under project number: 2021SABE007.

Author information

Authors and Affiliations

  1. School of Medicine, Department of Anatomy, Pamukkale University, 20070, Denizli, Turkey

    Sevval Ozturk, Ibrahim Cuneyit & Baris Ozgur Donmez

  2. School of Medicine, Department of Physiology, Pamukkale University, 20070, Denizli, Turkey

    Fatih Altuntas

  3. School of Medicine, Department of Medical Genetics, Pamukkale University, 20070, Denizli, Turkey

    Ege Riza Karagur

  4. School of Medicine, Department of Medical Biochemistry, Pamukkale University, 20070, Denizli, Turkey

    Aysegul Cort Donmez

  5. School of Dentistry, Department of Anatomy, Ankara University, 06650, Ankara, Turkey

    Mert Ocak

  6. School of Medicine, Department of Biophysics, Karamanoglu Mehmetbey University, 70200, Karaman, Turkey

    Mustafa Unal

  7. Faculty of Engineering, Department of Bioengineering, Karamanoglu Mehmetbey University, 70200, Karaman, Turkey

    Mustafa Unal

  8. Faculty of Engineering, Department of Mechanical Engineering, Ege University, 35040, Izmir, Turkey

    Mehmet Sarikanat

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  1. Sevval Ozturk
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Contributions

SO (Conceptualization, Methodology, Investigation, Visualization, Formal Analysis), IC (Investigation), FA (Investigation), ERK (Investigation, Writing-Review and Editing, Visualization), ACD (Writing-Review and Editing, Investigation), MO (Formal analysis, Investigation, Visualization), MU (Writing-Review and Editing, Investigation, Visualization), MS (Investigation), BOD (Writing-Review and Editing, Resources, Formal Analysis, Conceptualization, Project administration).

Corresponding author

Correspondence to Baris Ozgur Donmez.

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None.

Ethical approval

The study was approved by the Animal Experimentation Ethical Committee of Pamukkale University (protocol number: 60758568–020/48556).

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Ozturk, S., Cuneyit, I., Altuntas, F. et al. Resveratrol prevents ovariectomy-induced bone quality deterioration by improving the microarchitectural and biophysicochemical properties of bone. J Bone Miner Metab 41, 443–456 (2023). https://doi.org/10.1007/s00774-023-01416-z

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