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Anti-inflammatory potential of novel hexapeptide derived from Meretrix meretrix foot and its functional properties

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Abstract

The study aimed to identify bioactive peptide from Meretrix meretrix Linnaeus foot (MMF) and examine its potential of suppressing inflammation. In brief, the anti-inflammatory activity was identified by erythrocyte membrane protection and protein denaturation assay from MMF peptic 9th-h hydrolysate and was separated with three molecular weight cut-off units. The obtained four fractions were testified for activity and the fraction (10–3 kDa) with maximum activity was purified using gel permeation chromatography. Finally, the peptide sequence was identified as Asn–Pro–Ala–Gln–Asp–Cys (647.559 Da) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The hexapeptide was characterised for functional properties at different pH range. The non-toxic hexapeptide was able to reduce the cyclooxygenase (COX)-2 activation, pro-inflammatory cytokines and nitric oxide (NO) production significantly in RAW264.7 macrophage cells. The current results propose that the hexapeptide derived from MMF protein can act as an effective anti-inflammatory against pro-inflammatory cytokines, COX-2 and NO. Moreover, it could be used as an effective alternative source for drugs in pharma and also as an ingredient in food industries.

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Acknowledgements

We gratefully acknowledge to the management, SRM Institute of Science and Technology for providing the facilities.

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Authors and Affiliations

  1. Biopharmaceutical Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, 603 203, Tamil Nadu, India

    Ila Joshi & Rasool Abdul Nazeer

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  1. Ila Joshi
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  2. Rasool Abdul Nazeer
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Correspondence to Rasool Abdul Nazeer.

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Joshi, I., Nazeer, R.A. Anti-inflammatory potential of novel hexapeptide derived from Meretrix meretrix foot and its functional properties. Amino Acids 52, 1391–1401 (2020). https://doi.org/10.1007/s00726-020-02899-0

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