Abstract
The spectroscopy of electron paramagnetic resonance, unlike optical methods, allows observing paramagnetic molecules inside a non-paramagnetic matrix of any morphology, which makes it possible to investigate the behavior of proteins immobilized on porous carriers. In the present work the preferential orientation of lysozyme from chicken egg white, spin-labeled in position his-15, immobilized on the surface of a series of porous oxide carriers, has been defined by computer simulation of the dynamic EPR spectra.
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Acknowledgements
The work was supported by Russian Foundation for Basic Research (RFBR) (grant 19–33-50073 mol_nr and 18–29-12129mk) and in part by M.V. Lomonosov Moscow State University Program of Development. Authors are grateful to Prof. Vladimir P. Timofeev and Dr. Yaroslav V. Tkachev (Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Moscow) for a fruitful discussion and valuable advices.
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Antonov, D.O., Chumakova, N.A. & Kovaleva, E.G. Orientation of Spin-Labeled Lysozyme from Chicken Egg White Immobilized on Porous Oxide Carriers. Appl Magn Reson 51, 679–690 (2020). https://doi.org/10.1007/s00723-020-01231-z
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DOI: https://doi.org/10.1007/s00723-020-01231-z