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A comparative study of PNIPAM nanoparticles of curcumin, demethoxycurcumin, and bisdemethoxycurcumin and their effects on oxidative stress markers in experimental stroke

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Abstract

Oxidative stress and inflammatory damage play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. The development of new strategies for enhancing drug delivery to the brain is of great importance in diagnostics and therapeutics of central nervous diseases. The present study examined the hypothesis that intranasal delivery of nanoformulation of curcuminoids would reduce oxidative stress-associated brain injury after middle cerebral artery occlusion (MCAO). The rats were subjected to 2 h of MCAO followed by 22 h reperfusion, after which the grip strength, locomotor activity was performed. The effects of treatment in the rats were assessed by grip strength, locomotor activity and biochemical studies (glutathione peroxidase, glutathione reductase, lipid peroxidation, superoxide dismutase, and catalase) in the brain. Pretreatment with polymeric N-isopropyl acryl amide (PNIPAM) nanoparticles formulation of all three curcuminoids (curcumin (Cur), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC)) at doses (100 μg/kg body weight) given intranasally was effective in bringing significant changes on all the parameters. While nanoformulation of curcumin at a dose of 100 μg/kg body weight was most active in the treatment of cerebral ischemia as compared to others nanoformulation of curcuminoids. The potency of antioxidant activity significantly decreased in the order of PNIPAM nanoformulation of Cur > DMC >> BDMC, thus suggesting the critical role of methoxy groups on the phenyl ring.

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Acknowledgments

The authors are thankful to the Council for Scientific and Industrial Research, Pusa Road, New Delhi, for the granting CSIR-SRF which supported this study.

Conflict of interest

Authors have no conflict of interest in this paper. The author (Niyaz Ahmad) is grateful to the Council for Scientific and Industrial Research, Pusa Road, New Delhi, for financial grant of this research.

Animal study

Experiments were conducted in accordance with Hamdard Institutional Ethics Committee (JH/FP/0847/2012). Approval for the animal study was given by Institutional Animal Ethical Committee.

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Authors and Affiliations

  1. Nanomedicine lab, Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, 110062, India

    Niyaz Ahmad, Zeenat Iqbal & Farhan Jalees Ahmad

  2. Department of Medical Elemental and Toxicology, Faculty of Science, Hamdard University, New Delhi, 110062, India

    Sadiq Umar & Mohammad Ashafaq

  3. Department of Pharmacology, Faculty of Pharmacy, Hamdard University, New Delhi, 110062, India

    Mohd Akhtar

  4. Department of Chemistry, Faculty of Science, Hamdard University, New Delhi, 110062, India

    Mohd Samim

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  1. Niyaz Ahmad
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Correspondence to Farhan Jalees Ahmad.

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Handling Editor: Damjana Drobne

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Ahmad, N., Umar, S., Ashafaq, M. et al. A comparative study of PNIPAM nanoparticles of curcumin, demethoxycurcumin, and bisdemethoxycurcumin and their effects on oxidative stress markers in experimental stroke. Protoplasma 250, 1327–1338 (2013). https://doi.org/10.1007/s00709-013-0516-9

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  • DOI: https://doi.org/10.1007/s00709-013-0516-9

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