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Ectopic overexpression of vacuolar and apoplastic Catharanthus roseus peroxidases confers differential tolerance to salt and dehydration stress in transgenic tobacco

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Abstract

CrPrx and CrPrx1 are class III peroxidases previously cloned and characterized from Catharanthus roseus. CrPrx is known to be apoplastic in nature, while CrPrx1 is targeted to vacuoles. In order to study their role in planta, these two peroxidases were expressed in Nicotiana tabacum. The transformed plants exhibited increased peroxidase activity. Increased oxidative stress tolerance was also observed in transgenics when treated with H2O2 under strong light conditions. However, differential tolerance to salt and dehydration stress was observed during germination of T1 transgenic seeds. Under these stresses, the seed germination of CrPrx-transformed plants and wild-type plants was clearly suppressed, whereas CrPrx1 transgenic lines showed improved germination. CrPrx-transformed lines exhibited better cold tolerance than CrPrx1-transformed lines. These results indicate that vacuolar peroxidase plays an important role in salt and dehydration stress over cell wall-targeted peroxidase, while cell wall-targeted peroxidase renders cold stress tolerance.

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Acknowledgments

SK and MJ thank Council of Scientific and Industrial Research, India for providing fellowship. The authors thank Dr. Rekha Kushwaha for assisting in statistical analysis. This work is supported by the core grant of the National Institute of Plant Genome Research, New Delhi, India.

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The authors declare that they have no conflict of interest.

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  1. Santosh Kumar

    Present address: University of Kentucky, 1403, Veterans Drive, 3012 Plant Science Building, Room Number 434, Lexington, USA

Authors and Affiliations

  1. National Institute of Plant Genome Research, 10531, Aruna Asaf Ali Road, New Delhi, 110 067, India

    Santosh Kumar, Monika Jaggi & Alok Krishna Sinha

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  1. Santosh Kumar
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Correspondence to Alok Krishna Sinha.

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Kumar, S., Jaggi, M. & Sinha, A.K. Ectopic overexpression of vacuolar and apoplastic Catharanthus roseus peroxidases confers differential tolerance to salt and dehydration stress in transgenic tobacco. Protoplasma 249, 423–432 (2012). https://doi.org/10.1007/s00709-011-0294-1

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