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Subcellular localization of V2 protein of Tomato leaf curl Java virus by using green fluorescent protein and yeast hybrid system

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Abstract

Tomato leaf curl Java virus-A (ToLCJV-A[ID]) from Southeast Asia is a new member of the emerging group of monopartite begomoviruses that require a betasatellite component for symptom induction. Previously, we have elucidated the role of V1 ORF encoded by ToLCJV-A[ID] in cell-to-cell movement. In this study, the role of V2 (PreCP) in localization was determined. Subcellular localization of ToLCJV-A[ID] V2 in plant tissues showed that this protein is co-localized to the cell cytoplasm, perinuclear and associated with the endoplasmic reticulum network. The results obtained from deletion analysis indicate that fusion of N-terminal part of the V2, containing the nuclear export signals (NES), directed the accumulation of fluorescence towards the cell cytoplasm. Furthermore, functionality of the NES (20LAVKYLQLV29) in the N-terminal part of the V2 protein was confirmed by one-hybrid yeast system. Taken together, these results suggest that V2 enhances the coat protein-mediated nuclear export of ToLCJV-A[ID] and is consistent with the model in which V2 mediates viral DNA export from the nucleus to the plasmodesmata.

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Acknowledgments

We thank Drs V. Citovsky for the yeast one-hybrid vectors and Y. Niwa for the pGFP (S65T) plasmid and grateful to the Japan Society for the Promotion of Science for awarding the JSPS Postdoctoral Fellowship to conduct this research. We are grateful to Prof. HL Sharma and N. Sharma (HPKV, Palampur) for critical reading of the manuscript and Project Director, DWR, Karnal for encouragement while preparing this manuscript.

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The authors declare that they have no conflict of interest.

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Correspondence to Pradeep Sharma.

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Sharma, P., Gaur, R.K. & Ikegami, M. Subcellular localization of V2 protein of Tomato leaf curl Java virus by using green fluorescent protein and yeast hybrid system. Protoplasma 248, 281–288 (2011). https://doi.org/10.1007/s00709-010-0166-0

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  • DOI: https://doi.org/10.1007/s00709-010-0166-0

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