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Light-induced decrease in DCF fluorescence of wheat leaves in the presence of salicyl hydroxamate

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Summary.

5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA), a permeative indicator of oxidative stress, was loaded into dissected leaves of wheat in order to monitor the temporal development of reactive oxygen species. DCF fluorescence was found to be constant under dark conditions. Upon loading the leaves with salicyl hydroxamate, a blocker of the alternative oxidase, DCF fluorescence linearly increased in the dark. This indicates a function of alternative oxidase in preventing reactive oxygen radicals in the mitochondria. Upon illumination, the DCF signal decreased within 5 min. As illuminated chloroplasts would increase the load of reactive oxygen species, the observed decrease cannot be assigned to the production of reactive oxygen species in the chloroplasts. Three different putative mechanisms are considered which all assign an important role to light-induced delivery of NAD(P)H: (1) direct quenching of DCF fluorescence by light-generated NAD(P)H, (2) light-stimulated activation of scavenging enzymes, or (3) redirection of mitochondrial electron fluxes as caused by the delivery of excess redox equivalents (NADH) from the chloroplasts.

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Abbreviations

AOX:

alternative oxidase

DCF-DA:

5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate

ROS:

reactive oxygen species

SHAM:

salicyl hydroxamate

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Authors and Affiliations

  1. Center of Biochemistry and Molecular Biology, Christian-Albrechts-Universität zu Kiel, Kiel

    E. Hammes, A. Hoffmann, C. Plieth & U.-P. Hansen

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  1. E. Hammes
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  2. A. Hoffmann
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  3. C. Plieth
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  4. U.-P. Hansen
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Correspondence to U.-P. Hansen.

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Correspondence and reprints: Center of Biochemistry and Molecular Biology, Christian-Albrechts-Universität zu Kiel, Leibnizstrasse 11, 24098 Kiel, Federal Republic of Germany.

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Hammes, E., Hoffmann, A., Plieth, C. et al. Light-induced decrease in DCF fluorescence of wheat leaves in the presence of salicyl hydroxamate. Protoplasma 227, 11–15 (2005). https://doi.org/10.1007/s00709-005-0134-2

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  • DOI: https://doi.org/10.1007/s00709-005-0134-2

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