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Identification and molecular characterization of two novel viruses from Allamanda cathartica in China

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Abstract

Allamanda cathartica is an ornamental medicinal plant that grows widely in the tropics. In the present study, two novel viruses, Allamanda chlorotic virus A (AlCVA) and Allamanda chlorotic virus B (AlCVB), were identified in an A. cathartica plant with interveinal chlorosis by ribosomal RNA-depleted total-RNA sequencing. Phylogenetic analysis and sequence comparisons confirmed that AlCVA and AlCVB belong to the families Closteroviridae and Betaflexiviridae, respectively. Long, flexuous, filamentous virus particles approximately 12 nm in diameter and 784-2291 nm in length were observed using transmission electron microscopy. A specific RT-PCR assay was used to demonstrate a consistent association of viral infection with symptoms.

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All data supporting the findings of this study are available within the paper and its supplementary information.

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Acknowledgements

We thank Liu Jia-liang (Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences) for the technical assistance help with transmission electron microscopy.

Funding

This work was supported by the National Natural Science Foundation of China (32102182) and the Central Public-Interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (1630042019001).

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Authors and Affiliations

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Haiyan Che, Yuxin Ma & Haibo Long

  2. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, China

    Haiyan Che & Haibo Long

  3. School of Life and Health Sciences, Hainan University, Haikou, 570228, China

    Yangxin Wu

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  1. Haiyan Che
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  2. Yuxin Ma
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  4. Haibo Long
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Correspondence to Haibo Long.

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Che, H., Ma, Y., Wu, Y. et al. Identification and molecular characterization of two novel viruses from Allamanda cathartica in China. Arch Virol 169, 124 (2024). https://doi.org/10.1007/s00705-024-06041-3

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  • DOI: https://doi.org/10.1007/s00705-024-06041-3

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