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A novel alternavirus with three dsRNA segments from Fusarium pseudograminearum, the pathogen of Fusarium crown rot in wheat

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Abstract

Three dsRNA segments were detected in Fusarium pseudograminearum strain CF14029, a pathogen causing Fusarium crown rot in China. Characterization and sequence analysis confirmed that these dsRNA sequences originated from the same virus. The viral genome consists of three dsRNA segments: dsRNA1 (3,560 nt in length), encoding an RNA-dependent RNA polymerase (RdRp), dsRNA2 (2,544 nt in length), encoding a hypothetical protein, and dsRNA3 (2,478 nt in length), encoding a putative coat protein (CP). Phylogenetic analysis based on the RdRp and CP amino acid sequences revealed a high degree of similarity of this virus to members of the genus Alternavirus, family Alternaviridae, isolated from other Fusarium fungi. As a novel member of the genus Alternavirus, this virus was provisionally named "Fusarium pseudograminearum alternavirus 1" (FpgAV1). Like other alternaviruses found in Fusarium species, the positive-sense strand of each genomic dsRNA of FpgAV1 possesses a poly(A) tail and a distinctive 5'-terminal octamer sequence (5’-GCT GTG TG-3’). This is the first report of the genomic sequence of an alternavirus identified in F. pseudograminearum.

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Data availability

The complete genome sequence of FpgAV1 has been deposited in the NCBI database with the accession numbers OR754230, OR754231, and OR754232.

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Funding

This work was supported by the National Key R&D Program of China (grant no. 2022YFD1400105), the National Natural Science Foundation of China (grant no. 32072375), and the earmarked fund of the China Agriculture Research System (CARS-3-34).

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Authors and Affiliations

  1. College of Agriculture, Yangtze University, 434025, Hubei, Jingzhou, China

    Fuyu Liu & Qingchao Deng

  2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, China

    Fuyu Liu, Shulin Cao, Haiyan Sun, Huaigu Chen & Wei Li

  3. College of Plant Protection, Nanjing Agricultural University, 210095, Jiangsu, Nanjing, China

    Lele Xia & Wei Li

Authors
  1. Fuyu Liu
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  2. Lele Xia
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  3. Shulin Cao
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  4. Haiyan Sun
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  5. Huaigu Chen
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  6. Qingchao Deng
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  7. Wei Li
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Lele Xia, Shulin Cao, and Haiyan Sun. The first draft of the manuscript was written by Fuyu Liu, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Qingchao Deng or Wei Li.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Ioly Kotta-Loizou

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Liu, F., Xia, L., Cao, S. et al. A novel alternavirus with three dsRNA segments from Fusarium pseudograminearum, the pathogen of Fusarium crown rot in wheat. Arch Virol 169, 49 (2024). https://doi.org/10.1007/s00705-024-05982-z

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