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Genomic and biological characteristics of a novel lytic phage, vB_MscM-PMS3, infecting the opportunistic zoonotic pathogen Mammaliicoccus sciuri

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Abstract

Mammaliicoccus sciuri is an opportunistic zoonotic pathogen in humans and animals. We isolated the Mammaliicoccus phage vB_MscM-PMS3, which was also able to infect and lyse M. sciuri and M. lentus. The phage genome is a linear dsDNA that is 147,811 bp in length and contains 206 ORFs and three tRNA genes. It showed low genome coverage (< 17%) and sequence identity (< 91.3%) to other phage genomes. Phylogenetic analysis based on the whole genome and major capsid protein revealed that this phage clustered with members of the subfamily Twortvirinae of the family Herelleviridae, but it was distinctly separated from the other members, indicating its uniqueness.

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Fig. 1
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Data availability

The complete genome sequence of phage vB_MscM-PMS3 has been deposited in the NCBI GenBank database under accession number MZ573780.2.

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Funding

This work was supported by the ‘Development of Technology for the Biomaterialization of Marine Fishery Byproducts’ of the Korea Institute of Marine Science and Technology Promotion [KIMST], funded by the Ministry of Oceans and Fisheries [KIMST-20220128], and also supported by a research grant from the National Institute of Biological Resources [NIBR202322101], funded by the Ministry of Environment of the Republic of Korea.

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Author notes

  1. Hyemin Kwon, Seon Young Park and Seungki Lee contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea

    Hyemin Kwon

  2. Division of Animal and Dairy Sciences, College of Agriculture and Life Science, Chungnam National University, Daejeon, 34134, Republic of Korea

    Seon Young Park

  3. Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

    Seungki Lee

  4. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea

    Se Chang Park

  5. Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 13120, Republic of Korea

    Ye Bin Kim & Ji Hyung Kim

  6. Laboratory of Aquatic Biomedicine, College of Veterinary Medicine & Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jee Eun Han

  7. Department of Biological Sciences, Kyonggi University, Suwon, 16227, Republic of Korea

    Sang Guen Kim

Authors
  1. Hyemin Kwon
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Contributions

JHK and JEH conceived the idea and designed the experiments. Material preparation and data collection were performed by HK, SYP, SL, and YBK. SGK and SCP helped with the experimental design. The first draft of the manuscript was written by HK, SYP, and SL, and all authors commented on previous versions of the manuscript and read and approved the final manuscript.

Corresponding authors

Correspondence to Jee Eun Han or Ji Hyung Kim.

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The authors declare that they have no conflict of interest.

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Kwon, H., Park, S.Y., Lee, S. et al. Genomic and biological characteristics of a novel lytic phage, vB_MscM-PMS3, infecting the opportunistic zoonotic pathogen Mammaliicoccus sciuri. Arch Virol 169, 4 (2024). https://doi.org/10.1007/s00705-023-05940-1

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  • DOI: https://doi.org/10.1007/s00705-023-05940-1

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