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Protective effects of the NLRP3 inflammasome against infectious bursal disease virus replication in DF-1 cells

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Abstract

Inflammatory responses are an important part of the innate immune response during viral infection. Various inflammasome complexes have been identified. The pyrin domain-containing 3 (NLRP3) inflammasome plays a critical role in detecting some RNA viruses, such as influenza virus. However, the effect of the NLRP3 inflammasome on infectious bursal disease virus (IBDV) replication is still unclear. Here, we report that IBDV-infection induces the transcription of NLRP3 inflammasome and IL-1β genes in the immortalized chicken embryo fibroblast cell line DF-1. Inhibition of caspase-1 by Belnacasan (VX-765) suppressed the transcription of IL-1β, reduced cell lysis, and significantly promoted IBDV replication in DF-1 cells. Furthermore, knockdown of NLRP3 by small interfering RNA promoted IBDV replication in the host cells. Thus, IBDV can induce NLRP3 inflammasome activation in DF-1 cells through a mechanism requiring viral replication, revealing a new antiviral mechanism employed by the host.

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Abbreviations

IBDV:

Infectious bursal disease virus

VX-765:

Belnacasan

LDH:

Lactate dehydrogenase

RNAi:

RNA interference

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Acknowledgements

The authors thank Drs. Jue Liu and Wenhai Feng for their generous assistance. This work was supported in part by grants from the National Natural Science Foundation of China (31402172) and the Beijing Municipal Commission of Education for Science and Technology Development (D161100001516001). The funders had no role in the study design, data collection and analysis, or in deciding to prepare or publish this manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China

    Zhiyuan He & Yulin Ma

  2. China Animal Husbandry Industry Co. Ltd, Beijing, 100070, China

    Dongxun Wu

  3. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Wenhai Feng

  4. Key Laboratory for Veterinary Biologics and Chemical Drugs of the Ministry of Agriculture, Zhongmu Institute of China Animal Husbandry Industry Co. Ltd, Haidian District, Beijing, 100095, China

    Jin Xiao

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  1. Zhiyuan He
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Correspondence to Jin Xiao.

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He, Z., Ma, Y., Wu, D. et al. Protective effects of the NLRP3 inflammasome against infectious bursal disease virus replication in DF-1 cells. Arch Virol 166, 1943–1950 (2021). https://doi.org/10.1007/s00705-021-05099-7

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  • DOI: https://doi.org/10.1007/s00705-021-05099-7

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