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A mitovirus isolated from the phytopathogenic fungus Alternaria brassicicola

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Abstract

Alternaria brassicicola is one of the causal agents of alternaria blackspot in rapeseed. In this study, a dsRNA segment was isolated and sequenced from the fungus. The complete nucleotide sequence of the dsRNA was 2506 bp in length and, using the fungal mitochondrial genetic code, was predicted to contain a single large open reading frame (ORF) in the positive strand. This ORF was predicted to encode a protein with 719 amino acids that contains characteristic conserved motifs of the RNA-dependent RNA polymerase (RdRp). BLAST analysis revealed that this protein had significant sequence similarity to the RdRp from viruses of the genus Mitovirus. These results indicated that the dsRNA segment represents the replicative form of a mitovirus, which is tentatively designated “Alternaria brassicicola mitovirus 1” (AbMV1) and is a new member of the genus Mitovirus in the family Narnaviridae.

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Acknowledgements

This study is supported by Excellent Doctoral Dissertation Cultivating Fund of Hunan Agricultural University (YB2015002).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People’s Republic of China

    Yi Chen, Hong Hong Shang, Bi Da Gao & Jie Zhong

  2. College of Biological Science and Technology, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People’s Republic of China

    Yi Chen & Hong Qi Yang

Authors
  1. Yi Chen
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  2. Hong Hong Shang
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  3. Hong Qi Yang
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  4. Bi Da Gao
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  5. Jie Zhong
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Correspondence to Bi Da Gao or Jie Zhong.

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Chen, Y., Shang, H.H., Yang, H.Q. et al. A mitovirus isolated from the phytopathogenic fungus Alternaria brassicicola . Arch Virol 162, 2869–2874 (2017). https://doi.org/10.1007/s00705-017-3371-8

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  • DOI: https://doi.org/10.1007/s00705-017-3371-8

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