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Dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 following modified live PRRSV vaccination in a PRRSV-infected herd

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Abstract

The objective of this study was to investigate the dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 following the use of a modified live PRRSV (MLV) vaccine. A PRRSV-positive farm with coexistence of types 1 and 2 and no history of MLV vaccination was investigated. Vaccination with a type 2 MLV (Ingelvac PRRS MLV, Boehringer Ingelheim, USA) was implemented. All sows were vaccinated at monthly intervals for two consecutive months and then every third month. Piglets were vaccinated once at 7-10 days of age and weaned to nursery facilities at 21-23 days of age. Serum samples were collected monthly before and after vaccination from four population groups, including replacement gilts and suckling, nursery and finishing pigs, and assayed by PCR. After a year of blood collection, amplified products were sequenced, resulting in 277 complete ORF5 gene sequences from 145 type 1 and 132 type 2 isolates. Prior to and following vaccination, both type 1 and type 2 PRRSV were isolated and found to coexist in an individual pig. Each genotype evolved separately without influencing the strain development of the other. Although the substitution rates of both genotypes were relatively similar, MLV vaccination appears to increase the heterogenicity of type 2 PRRSV, resulting in the emergence of three novel type 2 PRRSV clusters in the herd, including an MLV-like cluster, which disappeared within the month following whole-herd vaccination. Two additional clusters included one related to the MLV vaccine and one related to the endemic cluster of the herd.

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Acknowledgments

The authors are grateful to the Thailand Research Fund (Project Number MRG5080323 and IUG5080001) and the Government budget years 2008 and 2012 for funding this research.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Dachrit Nilubol, Thitima Tripipat & Tawatchai Hoonsuwan

  2. Genome Institute, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand

    Pavita Tipsombatboon & Jittima Piriyapongsa

Authors
  1. Dachrit Nilubol
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  2. Thitima Tripipat
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  3. Tawatchai Hoonsuwan
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  4. Pavita Tipsombatboon
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  5. Jittima Piriyapongsa
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Correspondence to Dachrit Nilubol.

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Nilubol, D., Tripipat, T., Hoonsuwan, T. et al. Dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 following modified live PRRSV vaccination in a PRRSV-infected herd. Arch Virol 159, 17–27 (2014). https://doi.org/10.1007/s00705-013-1781-9

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  • DOI: https://doi.org/10.1007/s00705-013-1781-9

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