Abstract
In foot-and-mouth disease (FMD) control programme, liquid-phase blocking ELISA (LPBE) is widely used to assay vaccine-induced seroconversion. Currently, the assay utilizes inactivated FMD virus antigen for the detection of antibodies in serum samples. To develop a non-infectious substitute for the antigen in LPBE, we expressed the structural polypeptide of FMDV (serotype A) using a baculovirus expression system, and show that inclusion of viral 3C with reduced protease activity resulted in a higher yield of structural proteins. Structural proteins expressed in insect cells assembled into empty virus-like particles (VLPs) and showed antigenicity comparable to chemically inactivated FMDV. Screening of serum samples from FMD-vaccinated cattle showed that the test performance of VLP-LPBE had a correlation of 0.89 with conventional inactivated virus antigen LPBE. The VLP-LPBE developed here demonstrates the diagnostic application of recombinant FMDV VLPs in monitoring seroconversion following FMD vaccination.
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Acknowledgment
We are grateful to the Director, Indian Veterinary Research Institute (IVRI), Izatnagar, for facilitating this work. We also thank Dr. K.P. Suresh of Project Directorate on Animal Disease Monitoring and Surveillance, Bangalore, for helpful discussions on statistical analysis.
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S. H. Basagoudanavar and M. Hosamani contributed equally
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Basagoudanavar, S.H., Hosamani, M., Tamil Selvan, R.P. et al. Development of a liquid-phase blocking ELISA based on foot-and-mouth disease virus empty capsid antigen for seromonitoring vaccinated animals. Arch Virol 158, 993–1001 (2013). https://doi.org/10.1007/s00705-012-1567-5
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DOI: https://doi.org/10.1007/s00705-012-1567-5