Abstract
TMVOhioV was first described 1969 by [1] because it did break resistance of tomato breeding lines containing Tm-1- and Tm-2 resistance genes. It was obtained 1987 from Wetter (Saarbrücken, Germany) and transferred into the DSMZ-Plant Virus Collection (Braunschweig, Germany). A partial sequence of TMVOhioV, the CP gene, has been reported [11] and its comparison with a TMV type isolates (TMVtype), e.g. EMBL: V01409, revealed 50 point mutations in a total of 477 nucleotides (nts) leading to the replacement of only 7 amino acids (aa). In order to investigate the mutations in the non-translated regions and the number of silent mutation in the three other open reading frames (ORF), we sequenced the complete genome of isolate TMVOhioV and compared it to those of other Tobamoviruses.
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Acknowledgments
The supply of the original virus material by the DSMZ is gratefully acknowledged. P.W. was supported by The Hamburger Senat für Arbeit und Soziales.
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705_2011_1171_MOESM1_ESM.jpg
Fig. 1 SOM Phylogenetic tree showing the position of Ohio V among accepted tobacco mosaic viruses based on complete nucleotide sequences. The sequences were aligned by Clustal X version 2.1.10, and the phylogenetic tree was generated with MEGA (version 4.0) by the NJ-method with 1000 bootstrap replicates (bootstrap values greater than 70% are shown). Tobacco rattle virus (TRV, BAA00110) was used as an outgroup. TMVRussia (NC001367); TMVV2 (V01409); TMVKorea (X68110); TMVChina (AF165190); TMVUSA (AF273221); TMVFujian (AF395127); TMV017 (AF395128); TMV152 (AF395129); TMVpet-TW (EF392659) (JPEG 37 kb).
705_2011_1171_MOESM2_ESM.docx
Fig. 2 SOM Visualisation of silent mutations in an alignment of the movement protein ORFs of Ohio V and a TMVtype strain including their amino acid sequences. The nucleotide sequences were aligned with their translational products and then manually stacked for the two different isolates. Synonymous mutations are marked in red to differentiate them from non-synonymous mutations, which are marked in blue (Doc 15.5 kb).
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Körbelin, J., Willingmann, P., Adam, G. et al. The complete sequence of tobacco mosaic virus isolate Ohio V reveals a high accumulation of silent mutations in all open reading frames. Arch Virol 157, 387–389 (2012). https://doi.org/10.1007/s00705-011-1171-0
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DOI: https://doi.org/10.1007/s00705-011-1171-0