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CPm gene diversity in field isolates of Citrus tristeza virus from Colombia

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Abstract

The nucleotide sequence diversity of the CPm gene from 28 field isolates of Citrus tristeza virus (CTV) was assessed by SSCP and sequence analyses. These isolates showed two major shared haplotypes, which differed in distribution: A1 was the major haplotype in 23 isolates from different geographic regions, whereas R1 was found in isolates from a discrete region. Phylogenetic reconstruction clustered A1 within an independent group, while R1 was grouped with mild isolates T30 from Florida and T385 from Spain. Some isolates contained several minor haplotypes, which were very similar to, and associated with, the major haplotype.

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References

  1. Albiach-Martí MR, Mawassi M, Gowda S, Satyanarayana T, Hilf ME, Shanker S, Almira EC, Vives MC, López C, Guerri J, Florez R, Moreno P, Garnsey SM, Dawson WO (2000) Sequences of Citrus tristeza virus separated in time and space are essentially identical. J Virol 74:6856–6865

    Article  PubMed  Google Scholar 

  2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) BLAST: basic local alignment search tool. J Mol Biol 215:403–410

    CAS  PubMed  Google Scholar 

  3. Ayllón MA, Rubio L, Moya A, Guerri J, Moreno P (1999) The haplotype distribution of two genes of Citrus tristeza virus is altered after host change of aphid transmission. Virology 255:32–39

    Article  PubMed  Google Scholar 

  4. Cambra M, Camarasa E, Gorris MT, Garnsey SM, Carbonell E (1991) Comparison of different immunosorbent assays for Citrus tristeza virus (CTV) using CTV-specific monoclonal and polyclonal antibodies. In: Brlansky RH, Lee RF, Timmer LW (eds) Proceedings of the 11th conference International Organization of Citrus Virologists. IOCV, Riverside, pp 38–45

    Google Scholar 

  5. Domingo E, Holland JJ (1997) RNA virus mutations and fitness for survival. Annu Rev Microbiol 51:151–178

    Article  CAS  PubMed  Google Scholar 

  6. Febres VJ, Ashoulin L, Mawassi M, Frank A, Bar-Joseph M, Manjunath KL, Lee RF, Niblett CL (1996) The p27 protein is present at one end of Citrus tristeza virus particles. Phytopathology 86:1331–1335

    CAS  Google Scholar 

  7. García-Arenal F, Fraile A, Malpica JM (2001) Variability and genetic structure of plant virus populations. Annu Rev Phytopathol 39:157–186

    Article  PubMed  Google Scholar 

  8. Hall JS, French R, Morris TJ, Stenger DC (2001) Structure and temporal dynamics of populations within Wheat streak mosaic virus isolates. J Virol 75:10231–10243

    Article  CAS  PubMed  Google Scholar 

  9. Iglesias NG, Gago-Zachert SP, Robledo G, Costa N, Plata MI, Vera O, Grau O, Semorile LC (2008) Population structure of Citrus tristeza virus from field Argentinean isolates. Virus Genes 36:199–207

    Article  CAS  PubMed  Google Scholar 

  10. Karasev AV, Boyko VP, Gowda S, Nikolaeva OV, Hilf ME, Koonin EV, Niblett CL, Cline K, Gumpf DJ, Lee RF, Garnsey SM, Lewandowski DJ, Dawson WO (1995) Complete sequence of the Citrus tristeza virus RNA genome. Virology 208:511–520

    Article  CAS  PubMed  Google Scholar 

  11. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  12. Kong P, Rubio L, Polek M, Falk BW (2000) Population structure and genetic diversity of California Citrus tristeza virus (CTV) isolates. Virus Genes 21:139–145

    Article  CAS  PubMed  Google Scholar 

  13. Marroquin C, Olmos A, Gorris MT, Bertolini E, Martinez MC, Carbonell EA, Hermoso de Mendoza A, Cambra M (2004) Estimation of the number of aphids carrying Citrus tristeza virus that visit adult citrus trees. Virus Res 100:101–108

    Article  CAS  PubMed  Google Scholar 

  14. Moreno P, Guerri J, Muñoz N (1990) Identification of Spanish strains of Citrus tristeza virus by analysis of double-stranded RNA. Phytopathology 80:477–482

    Article  CAS  Google Scholar 

  15. Moreno P, Ambrós S, Albiach-Martí MR, Guerri J, Peña L (2008) Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Mol Plant Pathol 9:251–268

    Article  CAS  PubMed  Google Scholar 

  16. Nei M, Gojobori T (1986) Simple methods of estimating the number of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol 3:418–426

    CAS  PubMed  Google Scholar 

  17. Orita M, Suzuki Y, Sekiya T, Hayashi K (1989) Rapid and sensitive detection of point mutations and DNA polymorphisms. Genomics 5:874–879

    Article  CAS  PubMed  Google Scholar 

  18. Pappu HR, Pappu SS, Niblett CL, Lee RF, Civerolo EL (1993) Comparative sequence analysis of coat protein of biologically distinct Citrus tristeza closterovirus isolates. Virus Genes 7:255–264

    Article  CAS  PubMed  Google Scholar 

  19. Pappu HR, Karasev AV, Anderson EJ, Pappu SS, Hilf ME, Febres VJ, Eckloff RMG, McCaffery M, Boyko V, Gowda S, Dolja VV, Konin EV, Gumpf DJ, Cline KC, Garnsey SM, Dawson WO, Lee RF, Niblett CL (1994) Nucleotide sequence and organization of eight 3′ open reading frames of the Citrus tristeza closterovirus genome. Virology 199:35–46

    Article  CAS  PubMed  Google Scholar 

  20. Peñaranda J, Acosta O, Guzman-Barney M, Alegria A, Pappu HR, Pappu SS, Manjunath KL, Febres VJ, Lee RF, Niblett CL (1996) Incidence and characterization of mild and severe isolates of Citrus tristeza virus from Colombia. In: da Graça JV, Lee RF, Yokomi RK (eds) Proceedings of the 13th Conference International Organization of Citrus Virologists. IOCV, Riverside, pp 71–76

    Google Scholar 

  21. Permar TA, Garnsey SM, Gumpf DJ, Lee RF (1990) A monoclonal antibody that discriminates strains of Citrus tristeza virus. Phytopathology 80:224–228

    Article  Google Scholar 

  22. Rubio L, Ayllón MA, Kong P, Fernández A, Polek M, Guerri J, Moreno P, Falk BW (2001) Genetic variation of Citrus tristeza virus isolates from California and Spain: Evidence for mixed infections and recombination. J Virol 75:8054–8062

    Article  CAS  PubMed  Google Scholar 

  23. Sambrook J, Fritsch EF, Maniatis T (2000) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  24. Satyanarayana T, Gowda S, Ayllón MA, Dawson WO (2004) Closterovirus bipolar virion: evidence for initiation assembly by minor coat protein and its restriction to the genomic RNA 5′ region. Proc Natl Acad Sci USA 101:799–804

    Article  CAS  PubMed  Google Scholar 

  25. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. doi:10.1093/molbev/msm092

  26. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

O. Oliveros-Garay was a recipient of a Colciencias PhD scholarship. The authors would like to thank Colciencias for partially financing this research (project code 1101-12-652) and also Universidad Nacional de Colombia (project codes 20201006310 and 8001002).

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Authors and Affiliations

  1. Facultad de Agronomía, Universidad Nacional de Colombia, AA 14490, Bogotá, Colombia

    Oscar Arturo Oliveros-Garay & Natalhie Martinez-Salazar

  2. Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia

    Oscar Arturo Oliveros-Garay & Orlando Acosta

  3. Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia

    Yanneth Torres-Ruiz & Orlando Acosta

Authors
  1. Oscar Arturo Oliveros-Garay
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  2. Natalhie Martinez-Salazar
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  3. Yanneth Torres-Ruiz
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  4. Orlando Acosta
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Correspondence to Oscar Arturo Oliveros-Garay.

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In memoriam of José Peñaranda Valverde.

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Oliveros-Garay, O.A., Martinez-Salazar, N., Torres-Ruiz, Y. et al. CPm gene diversity in field isolates of Citrus tristeza virus from Colombia. Arch Virol 154, 1933–1937 (2009). https://doi.org/10.1007/s00705-009-0530-6

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  • DOI: https://doi.org/10.1007/s00705-009-0530-6

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