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Maximum likelihood and Bayesian analyses of a combined nucleotide sequence dataset for genetic characterization of a novel pestivirus, SVA/cont-08

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Abstract

Bovine viral diarrhoea virus 1 (BVDV-1) and Bovine viral diarrhoea virus 2 (BVDV-2) are two recognised bovine pestivirus species of the genus Pestivirus. Recently, a pestivirus, termed SVA/cont-08, was detected in a batch of contaminated foetal calf serum originating from South America. Comparative sequence analysis showed that the SVA/cont-08 virus shares 15–28% higher sequence identity to pestivirus D32/00_‘HoBi’ than to members of BVDV-1 and BVDV-2. In order to reveal the phylogenetic relationship of SVA/cont-08 with other pestiviruses, a molecular dataset of 30 pestiviruses and 1,896 characters, comprising the 5′UTR, Npro and E2 gene regions, was analysed by two methods: maximum likelihood and Bayesian approach. An identical, well-supported tree topology was observed, where four pestiviruses (SVA/cont-08, D32/00_‘HoBi’, CH-KaHo/cont, and Th/04_KhonKaen) formed a monophyletic clade that is closely related to the BVDV-1 and BVDV-2 clades. The strategy applied in this study is useful for classifying novel pestiviruses in the future.

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Acknowledgments

We are grateful to colleagues in the routine laboratory for providing the contaminated FCS used in this study.

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Authors and Affiliations

  1. Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 751 89, Uppsala, Sweden

    Lihong Liu, Hongyan Xia & Sándor Belák

  2. Joint R&D Division in Virology, The National Veterinary Institute, The Swedish University of Agricultural Sciences, 751 89, Uppsala, Sweden

    Lihong Liu, Hongyan Xia, Claudia Baule & Sándor Belák

  3. Department of Virology, Immunobiology and Parasitology (VIP), The National Veterinary Institute (SVA), 751 89, Uppsala, Sweden

    Lihong Liu

Authors
  1. Lihong Liu
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  2. Hongyan Xia
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  3. Claudia Baule
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  4. Sándor Belák
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Corresponding author

Correspondence to Lihong Liu.

Additional information

L. Liu and H. Xia contributed equally to this paper.

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Liu, L., Xia, H., Baule, C. et al. Maximum likelihood and Bayesian analyses of a combined nucleotide sequence dataset for genetic characterization of a novel pestivirus, SVA/cont-08. Arch Virol 154, 1111–1116 (2009). https://doi.org/10.1007/s00705-009-0419-4

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  • DOI: https://doi.org/10.1007/s00705-009-0419-4

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