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Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission

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Abstract

Deduced amino acid sequence and phylogenetic analyses of a group A rotavirus G9P[6] strain (designated as mcs/13-07), detected from a 3-year-old child in Eastern India, revealed a VP8* closely related to porcine P[6] strains (P[6] sublineage 1D), and the VP7 clustered with G9 lineage-III strains. To our knowledge, this is the first report of human P[6] strain clustering in sublineage Id. Thus, to further characterize the evolutionary diversity of strain mcs/13-07, all gene segments were analyzed. VP6 and NSP4 exhibited genetic relatedness to Wa-like human subgroup II strains, while VP1-3, NSP1-3 and NSP5 were closely related to porcine strains. Based on the new classification system of rotaviruses, mcs/13-07 revealed a G9–P[6]–I1–R1–C1–M1–A8–N1–T1–E1–H1 genotype with close similarity to human Wa-like and porcine Gottfried strains. Therefore, considering the porcine-like or porcine origin of multiple gene segments, it might be tempting to assume that strain mcs/13-07 represents a rare instance of whole-virus transmission from pig to human, after which the virus evolved with time. Alternatively, it is possible that strain mcs/13-07 resulted from multiple reassortment events involving human subgroup II and porcine P[6] strains. Nevertheless, detection of strain mcs/13-07 provides further evidence for complex interspecies transmission events, which are frequent in developing countries.

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Acknowledgments

This study was supported by financial assistance from Indian Council of Medical Research (ICMR), New Delhi, and the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases (Okayama University-NICED, India) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. A. Mukherjee and D. Dutta were supported by a Research Assistantship and Junior Research Fellowship, from ICMR and University Grant Commission, Govt. of India, respectively.

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Authors and Affiliations

  1. Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road Scheme XM, Beliaghata, Kolkata, 700010, West Bengal, India

    Anupam Mukherjee, Dipanjan Dutta, Parikshit Bagchi, Shiladitya Chattopadhyay, Triveni Krishnan, Trailokya Nath Naik & Mamta Chawla-Sarkar

  2. Department of Hygeine, Sapporo Medical University, S-1 W-17, Chuo-ku, Sapporo, 060-8556, Japan

    Souvik Ghosh, Shigeo Nagashima & Nobumichi Kobayashi

  3. Division of Clinical Medicine, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road Scheme XM, Beliaghata, Kolkata, 700010, West Bengal, India

    Phalguni Dutta

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  1. Anupam Mukherjee
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Correspondence to Mamta Chawla-Sarkar.

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Mukherjee, A., Dutta, D., Ghosh, S. et al. Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission. Arch Virol 154, 733–746 (2009). https://doi.org/10.1007/s00705-009-0363-3

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