Skip to main content
SpringerLink
Log in

Lack of vertical transmission of Hantaan virus from persistently infected dam to progeny in laboratory mice

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

It is unclear how the hantaviruses are transferred from infected to uninfected rodents. We studied the status of persistently infected laboratory mice and examined the frequency of viral transmission to their offspring. Expression of Hantaan virus nucleocapsid protein was detected in the lungs of persistently infected dams. None of the progeny displayed viral antigen, although they were strongly positive for IgG antibodies against hantavirus. There was neither hantavirus RNA nor virus-specific IgM antibodies or virus-specific CD8+ T cells in the progeny. These results did not show any indication for a vertical transmission of hantaviruses, at least in the laboratory mouse model studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Araki K, Yoshimatsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J (2001) Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections. J Clin Microbiol 39:2397–2404

    Article  PubMed  CAS  Google Scholar 

  2. Araki K, Yoshimatsu K, Lee BH, Kariwa H, Takashima I, Arikawa J (2003) Hantavirus-specific CD8(+)-T-cell responses in newborn mice persistently infected with Hantaan virus. J Virol 77:8408–8417

    Article  PubMed  CAS  Google Scholar 

  3. Araki K, Yoshimatsu K, Lee BH, Okumura M, Kariwa H, Takashima I, Arikawa J (2004) Age-dependent hantavirus-specific CD8(+) T-cell responses in mice infected with Hantaan virus. Arch Virol 149:1373–1382

    Article  PubMed  CAS  Google Scholar 

  4. Arikawa J, Ito M, Yao JS, Kariwa H, Takashima I, Hashimoto N (1994) Epizootiological studies of hantavirus infection among urban rats in Hokkaido, Japan: evidences for the persistent infection from the sero-epizootiological surveys and antigenic characterizations of hantavirus isolates. J Vet Med Sci 56:27–32

    PubMed  CAS  Google Scholar 

  5. Botten J, Mirowsky K, Kusewitt D, Ye C, Gottlieb K, Prescott J, Hjelle B (2003) Persistent Sin Nombre virus infection in the deer mouse (Peromyscus maniculatus) model: sites of replication and strand-specific expression. J Virol 77:1540–1550

    Article  PubMed  CAS  Google Scholar 

  6. Childs JE, Ksiazek TG, Spiropoulou CF, Krebs JW, Morzunov S, Maupin GO, Gage KL, Rollin PE, Sarisky J, Enscore RE et al (1994) Serologic and genetic identification of Peromyscus maniculatus as the primary rodent reservoir for a new hantavirus in the southwestern United States. J Infect Dis 169:1271–1280

    PubMed  CAS  Google Scholar 

  7. Compton SR, Jacoby RO, Paturzo FX, Smith AL (2004) Persistent Seoul virus infection in Lewis rats. Arch Virol 149:1325–1339

    Article  PubMed  CAS  Google Scholar 

  8. Dohmae K, Koshimizu U, Nishimune Y (1993) In utero and mammary transfer of hantavirus antibody from dams to infant rats. Lab Anim Sci 43:557–561

    PubMed  CAS  Google Scholar 

  9. Dohmae K, Okabe M, Nishimune Y (1994) Experimental transmission of hantavirus infection in laboratory rats. J Infect Dis 170:1589–1592

    PubMed  CAS  Google Scholar 

  10. Dohmae K, Nishimune Y (1995) Protection against hantavirus infection by dam’s immunity transferred vertically to neonates. Arch Virol 140:165–172

    Article  PubMed  CAS  Google Scholar 

  11. Harty JT, Badovinac VP (2008) Shaping and reshaping CD8+ T-cell memory. Nat Rev Immunol 8:107–119

    Article  PubMed  CAS  Google Scholar 

  12. Hutchinson KL, Rollin PE, Peters CJ (1998) Pathogenesis of a North American hantavirus, Black Creek Canal virus, in experimentally infected Sigmodon hispidus. Am J Trop Med Hyg 59:58–65

    PubMed  CAS  Google Scholar 

  13. Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M (2007) Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci USA 104:3213–3218

    Article  PubMed  CAS  Google Scholar 

  14. Kallio ER, Poikonen A, Vaheri A, Vapalahti O, Henttonen H, Koskela E, Mappes T (2006) Maternal antibodies postpone hantavirus infection and enhance individual breeding success. Proc Biol Sci 273:2771–2776

    Article  PubMed  Google Scholar 

  15. Lee HW, Lee PW, Baek LJ, Song CK, Seong IW (1981) Intraspecific transmission of Hantaan virus, etiologic agent of Korean hemorrhagic fever, in the rodent Apodemus agrarius. Am J Trop Med Hyg 30:1106–1112

    PubMed  CAS  Google Scholar 

  16. Meyer BJ, Schmaljohn CS (2000) Persistent hantavirus infections: characteristics and mechanisms. Trends Microbiol 8:61–67

    Article  PubMed  CAS  Google Scholar 

  17. Morita C, Morikawa S, Sugiyama K, Komatsu T, Ueno H, Kitamura T (1993) Inability of a strain of Seoul virus to transmit itself vertically in rats. Jpn J Med Sci Biol 46:215–219

    PubMed  CAS  Google Scholar 

  18. Okumura M, Yoshimatsu K, Araki K, Lee BH, Asano A, Agui T, Arikawa J (2004) Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses. Arch Virol 149:2427–2434

    Article  PubMed  CAS  Google Scholar 

  19. Tanishita O, Takahashi Y, Okuno Y, Tamura M, Asada H, Dantas JR Jr, Yamanouchi T, Domae K, Kurata T, Yamanishi K (1986) Persistent infection of rats with haemorrhagic fever with renal syndrome virus and their antibody responses. J Gen Virol 67(Pt 12):2819–2824

    Article  PubMed  Google Scholar 

  20. Taruishi M, Yoshimatsu K, Araki K, Okumura M, Nakamura I, Kajino K, Arikawa J (2007) Analysis of the immune response of Hantaan virus nucleocapsid protein-specific CD8+ T cells in mice. Virology 365:292–301

    Article  PubMed  CAS  Google Scholar 

  21. Yoshimatsu K, Yoo YC, Yoshida R, Ishihara C, Azuma I, Arikawa J (1993) Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins. Arch Virol 130:365–376

    Article  PubMed  CAS  Google Scholar 

  22. Yoshimatsu K, Arikawa J, Tamura M, Yoshida R, Lundkvist A, Niklasson B, Kariwa H, Azuma I (1996) Characterization of the nucleocapsid protein of Hantaan virus strain 76-118 using monoclonal antibodies. J Gen Virol 77(Pt 4):695–704

    Article  PubMed  CAS  Google Scholar 

  23. Zhang XK, Takashima I, Hashimoto N (1988) Role of maternal antibody in protection from hemorrhagic fever with renal syndrome virus infection in rats. Arch Virol 103:253–265

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. H. Sawa and Dr. T. Kimura for their assistance with the confocal immunofluorescence microscope and cryostat. Takako Shibuya is thanked for technical help. This work was supported in part by a grant from the 21st Century COE Program of Excellence for Zoonosis Control and in part by Grants-in-Aid for Scientific Research and the Development of Science from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

Author information

Authors and Affiliations

  1. Institute for Animal Experimentation, Hokkaido University Graduate School of Medicine, Kita-ku, Kita-15, Nishi-7, Sapporo, 060-8638, Japan

    Midori Taruishi, Kumiko Yoshimatsu, Rei Hatsuse, Megumi Okumura, Ichiro Nakamura & Jiro Arikawa

Authors
  1. Midori Taruishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kumiko Yoshimatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rei Hatsuse
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Megumi Okumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ichiro Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jiro Arikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jiro Arikawa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taruishi, M., Yoshimatsu, K., Hatsuse, R. et al. Lack of vertical transmission of Hantaan virus from persistently infected dam to progeny in laboratory mice. Arch Virol 153, 1605–1609 (2008). https://doi.org/10.1007/s00705-008-0156-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-008-0156-0

Keywords

Search

Navigation