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The European species of Ribes subg. Ribes: population genetic testing of classical systematics

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Abstract

According to current directories, the European subgenus Ribes subg. Ribes includes 4 species: R. rubrum, R. petraeum, R. spicatum and R. multiflorum. This taxonomy was inferred from morphology. In this study, the hypothesis that these four species exist within the subgenus was tested by population genetic methods. Microsatellite analysis was used to evaluate 118 wild-collected clones from 8 regions of Europe. In total, 263 alleles were detected. The results of cluster analysis and the analysis of population structure supported the classical placement of these 4 species previously determined by morphological systematics. The highest levels of infraspecific genetic diversity were found in R. petraeum and R. spicatum. Initial differentiation was identified between clones of R. petraeum originating from the Giant Mountains and others from the High Tatras. An even higher degree of differentiation was found between R. spicatum collected in Sweden and specimens from Scotland. At the supraspecific level, the tested species formed two clusters. R. petraeum and R. multiflorum constitute a group of European mountain red currants. The habitat typical for the second cluster, involving R. spicatum and R. rubrum, is mainly alluvial plain in the North Atlantic area. These results represent a sound basis for further taxonomy of red currant infraspecific taxa, nothotaxa and cultivars.

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Availability of data and materials

The datasets generated and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This dialogue would never have come about without the friendly intervention of Dr Vojtěch Holubec. Hana Udavská archived the genetic material, which is the basis for it all, and performed biomolecular analyses with exemplary reliability. Jitka Kissling drove on the roads of Sweden and elsewhere. Josef Harčařík provided the material from the Giant Mountains. Warm thanks for all this assistance, which enabled the completion of this contribution. Moreover, the authors thank to both reviewers for careful reading of the manuscript and for their valuable suggestions. The biomolecular aspect of this work was supported by the Czech Ministry of Agriculture, project no: MZe-RO0418. Some of the clones were stored in a genebank at the Research & Breeding Institute of Pomology Holovousy Ltd. (VSÚOH CZ).

Funding

The study was supported by the Ministry of Agriculture of the Czech Republic, institutional support Mze-RO0418.

Author information

Authors and Affiliations

  1. Crop Research Institute, Drnovská 507, Prague 6 – Ruzyně, Czech Republic

    Leona Leišová-Svobodová

  2. Alenor Conservation Garden, 38433, Záblatí, Czech Republic

    Pascal Kissling

Authors
  1. Leona Leišová-Svobodová
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  2. Pascal Kissling
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Contributions

Both authors, LLS and PK, designed the project, collected and analysed the data and wrote and approved the manuscript. LLS was responsible for Ribes genotyping, and PK was responsible for Ribes phenotyping and taxonomy.

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Correspondence to Leona Leišová-Svobodová.

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The authors declare no conflict of interest.

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Handling Editor: Michal Ronikier.

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Supplementary Information

Information on electronic supplementary material

Information on electronic supplementary material

Online Resource 1. Table of the origin of the 118 clones analysed.

Online Resource 2. Description of all studied Ribes subg. Ribes species.

Online Resource 3. List of microsatellite markers and basic information about them.

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Leišová-Svobodová, L., Kissling, P. The European species of Ribes subg. Ribes: population genetic testing of classical systematics. Plant Syst Evol 307, 41 (2021). https://doi.org/10.1007/s00606-021-01763-2

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