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Interspecific and intergeneric hybridization in South American Rhamnaceae-Colletieae

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Abstract

In Gondwanic Discaria, phenotypically intermediate individuals exist between Discaria chacaye (G. Don) Tortosa and Discaria articulata (Phil.) Miers, and between D. chacaye and related Ochetophila trinervis (Gillies ex Hook. & Arn.) Poepp. ex Miers. We studied phenology, pollinators, parental crossability, morphological features, and variation patterns on neutral markers. Intermediates occur wherever parental taxa are sympatric or in close proximity. Flowering was synchronous in D. chacaye and D. articulata for 2 weeks. They also shared three to six pollinator species (depending on site). O. trinervis, which flowered later, occasionally overlapped with D. articulata, with which it shared two pollinators. Germinable seeds were obtained from hand pollinations only from crosses with D. articulata as paternal parent and D. chacaye as maternal parent. Sequencing of chloroplast DNA revealed only two haplotypes. One occurred in D. articulata, D. chacaye, and their intermediates, and the other in O. trinervis and its intermediate with D. chacaye. Only pure parentals had diagnostic alleles by isozymes, whereas intermediates mainly showed those of D. chacaye. Our results confirm intrageneric hybridization between D. chacaye and D. articulata, and intergeneric hybridization between D. chacaye and related O. trinervis.

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References

  • Premoli AC (1996) Allozyme polymorphisms, outcrossing rates, and hybridization of three Nothofagus species. Genetica 97:55–64

    Article  CAS  Google Scholar 

  • Aagesen L (1999) Phylogeny of the tribe Colletieae. Bot J Linn Soc 131:1–43

    Article  Google Scholar 

  • Aagesen L, Medan D, Kellermann J, Hilger HH (2005) Phylogeny of the tribe Colletieae (Rhamnaceae)—a sensitivity analysis of the plastid region trnL-trnF combined with morphology. Plant Syst Evol 250:197–214

    Article  CAS  Google Scholar 

  • Ackermann M, Achatz M, Weigend M (2008) Hybridization and crossability in Caiophora (Loasaceae subfam. Loasoideae): are interfertile species and inbred populations results of a recent radiation? Am J Bot 95:1109–1121

    Article  PubMed  Google Scholar 

  • Acosta MC, Premoli AC (2010) Evidence of chloroplast capture in South American Nothofagus (subgenus Nothofagus, Nothofagaceae). Mol Phylogenet Evol 54:235–242

    Article  PubMed  Google Scholar 

  • Agresti A (1996) An introduction to categorical data analysis. Wiley, New York

    Google Scholar 

  • Arbetman M, Premoli AC (2011) Oldies (but goldies!): extracting DNA from cryopreserved allozyme homogenates. J Hered 102:764–769

    Article  PubMed  CAS  Google Scholar 

  • Arnold ML (1997) Natural Hybridization and Evolution. Oxford University Press, Oxford

    Google Scholar 

  • Barulenga M, Stölting KN, Salzburger W, Muschick M, Meyer A (2006) Sympatric speciation in Nicaraguan crater lake cichlid fish. Nature 439:719–723

    Article  Google Scholar 

  • Chaia EE, Valverde C, Wall LG (2006) Local adaptation of Frankia isolates to different Discaria (Rhamnaceae) host species growing in Patagonia. Curr Microbiol 53:523–528

    Article  PubMed  CAS  Google Scholar 

  • Devoto M, Medan D, Montaldo NH (2005) Patterns of interaction between plants and pollinators along an environmental gradient. Oikos 109:461–472

    Article  Google Scholar 

  • Devoto M, Medan D, Roig-Alsina A, Montaldo NH (2009) Patterns of species turnover in plant-pollinator communities along a precipitation gradient in Patagonia (Argentina). Austral Ecol 34:848–857

    Article  Google Scholar 

  • Dumolin S, Demesure B, Petit RJ (1995) Inheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR method. Theor Appl Genet 91:1253–1256

    Article  CAS  Google Scholar 

  • Ellstrand NC, Whitkus R, Rieseberg LH (1996) Distribution of spontaneous slant sybrids. Proc Natl Acad USA 93:5090–5093

    Article  CAS  Google Scholar 

  • Gore PL, Potts BM, Volker PW, Megalos J (1990) Unilateral cross-incompatibility in Eucalyptus: the case of hybridization between E. globulus and E. nitens. Aust J Bot 38:383–394

    Article  Google Scholar 

  • Kameyama Y, Toyama M, Ohara M (2005) Hybrid origins and F1 dominance in the free-floating, sterile bladderwort, Utricularia australis f. australis (Lentibulariaceae). Am J Bot 92:469–476

    Article  PubMed  CAS  Google Scholar 

  • Kellermann J, Medan D, Aagesen L, Hilger HH (2005) Rehabilitation of Ochetophila Poepp. ex Endl. (Rhamnaceae: Colletieae). N Z J Bot 43:865–869

    Article  Google Scholar 

  • King JN, Dancik BP (1983) Inheritance and linkage of isozymes in white spruce (Picea glauca). Can J Genet Cytol 25:430

    Google Scholar 

  • Knobloch IW (1972) Intergeneric hybridization in flowering plants. Taxon 21:97–103

    Article  Google Scholar 

  • Marchelli P, Gallo LA (2004) The combined role of glaciation and hybridization in shaping the distribution of genetic variation in a Patagonian southern beech. J Biogeogr 31:451–460

    Article  Google Scholar 

  • Medan D (1991) Reproductive phenology, pollination biology, and gynoecium development in Discaria americana (Rhamnaceae). N Z J Bot 29:31–42

    Article  Google Scholar 

  • Medan D (1993) Breeding system and maternal success of a perennial hermaphrodite, Discaria americana (Rhamnaceae). N Z J Bot 31:175–184

    Article  Google Scholar 

  • Medan D (2003) Reproductive biology of the Andean shrub Discaria nana (Rhamnaceae). Plant Biol 5:94–102

    Article  Google Scholar 

  • Medan D, Aagesen L (1995) Comparative flower and fruit structure in the Colletieae (Rhamnaceae). Bot Jahrb Syst 117:531–564

    Google Scholar 

  • Medan D, Devoto M (2005) Reproductive ecology of a perennial outcrosser with a naturally dissected distribution. Plant Syst Evol 254:173–184

    Article  Google Scholar 

  • Medan D, Schirarend C (2004) Rhamnaceae. In: Kubitzki K (ed) The families and genera of vascular plants. VI. Flowering plants–Dicotyledons: Celastrales, Oxalidales, Rosales, Cornales, Ericales. Springer, Heidelberg pp 320–338

  • Medan D, Basilio AM, Devoto M (1999) Biología reproductiva de Discaria chacaye (Rhamnaceae: Colletieae) en el Parque Nacional Lanín, Neuquén. Resúmenes, 19 Reunión Argentina de Ecología, Tucumán, April 1999

  • Medan D, Kellermann J, Aagesen L, Hilger HH (2009) The validity of Ochetophila Poepp. ex Endl. (Rhamnaceae: Colletieae). Feddes Rep 120:218–220

    Article  Google Scholar 

  • Mitton J, Linhart Y, Sturgeon K, Hamrick J (1979) Allozyme polymorphisms detected in mature needle tissue of ponderosa pine. J Hered 70:86–89

    CAS  Google Scholar 

  • Morrell P, Rieseberg LH (1998) Molecular tests of the proposed diploid hybrid origin of Gilia achilleifolia (Polemoniaceae). Am J Bot 85:1439–1453

    Article  PubMed  CAS  Google Scholar 

  • Murphy RW, Sites JW Jr, Buth DG, Haufler CH (1996) Proteins: isozyme electrophoresis. In: Hillis DM, Moritz C, Mable BK (eds) Molecular systematics. Sinauer Associates, Sunderland, pp 51–120

    Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of inividuals. Genetics 89:583–590

    PubMed  CAS  Google Scholar 

  • Park KR, Pak JH, Seo BB (2003) Allozyme variation in Paraixeris: a test for the diploid hybrid origin of Paraixeris koidzumiana (Compositae). Bot Bull Acad Sin 44:13–122

    Google Scholar 

  • Petit RJ, Bodénès C, Ducousso A, Roussel G, Kremer A (2003) Hybridization as a mechanism of invasion in oaks. New Phytol 161:151–164

    Article  Google Scholar 

  • Premoli AC, Mathiasen P, Acosta MC, Ramos VA (2012) Phylogeographically concordant chloroplast DNA divergence in sympatric Nothofagus s.s. How deep can it be? New Phytol 193:261–275

    Article  PubMed  CAS  Google Scholar 

  • Ranker C, Haufler H, Soltis PS, Soltis DE (1989) Genetic evidence for allopolyploidy in the neotropical fern Hemionitis pinnatifida (Adiantaceae) and the reconstruction of an ancestral genome. Syst Bot 14:439–447

    Article  Google Scholar 

  • Raven PH (1976) Systematics and plant population biology. Syst Bot 1:284–316

    Article  Google Scholar 

  • Reyes MF, Gobbi ME, Chaia EE (2011) Reproductive ecology of Ochetophila trinervis in northwest Patagonia. Funct Plant Biol 38:720–727

    Article  Google Scholar 

  • Rieseberg LH, Ellstrand NC (1993) What can molecular and morphological markers tell us about plant hybridization? Crit Rev Plant Sci 12:213–241

    CAS  Google Scholar 

  • SIB (2012) Sistema de Información de Biodiversidad, Administración de Parques Nacionales http://www.sib.gov.ar. Accessed 19 Feb 2012

  • Soltis DE, Soltis PS (1989) Allopolyploid speciation in Tragopogon: insights from chloroplast DNA. Am J Bot 76:1119–1124

    Article  CAS  Google Scholar 

  • Stebbins GL (1950) Variation and evolution in plants. Columbia University Press, New York

    Google Scholar 

  • Stebbins GL (1959) The role of hybridization in evolution. Proc Am Phil Soc 103:231–251

    Google Scholar 

  • Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Tortosa RD (1983a) El género Discaria (Rhamnaceae). Bol Soc Argent Bot 22:301–335

    Google Scholar 

  • Tortosa RD (1983b) Una especie polimorfa de Discaria: D. chacaye (G. Don) comb. nov. (Rhamnaceae) y sus híbridos presuntivos. Parodiana 2:79–98

    Google Scholar 

  • Tortosa RD (1989) El género Colletia (Rhamnaceae). Parodiana 5:279–332

    Google Scholar 

  • Tortosa RD (1992) El complejo Retanilla-Talguenea-Trevoa (Rhamnaceae). Darwiniana 31:223–252

    Google Scholar 

  • Tortosa RD (1999) Rhamnaceae. In: Zuloaga FO, Morrone O (ed) Catálogo de las plantas vasculares de la República Argentina II (Monographs in systematic botany from the Missouri botanical garden 74) pp 974–981

  • Tortosa RD, Aagesen L, Tourn GM (1996) Morphological studies in the tribe Colletieae (Rhamnaceae): analysis of architecture and inflorescences. Bot J Linn Soc 122:353–367

    Google Scholar 

  • Williams EG, Rouse JL (1988) Disparate style lengths contribute to isolation of species in Rhododendron. Austral J Bot 36:183–191

    Article  Google Scholar 

  • Wood TE, Nakazato T (2009) Investigating species boundaries in the Giliopsis group of Ipomopsis (Polemoniaceae): strong discordance among molecular and morphological markers. Am J Bot 96:853–861

    Article  PubMed  Google Scholar 

  • Wright GT, Briggs JD (2000) Population survey and notes on the ecology of the Leafy Anchor Plant (Discaria nitida) in New South Wales. Unpublished report. New South Wales National Parks and Wildlife Service, Hurstville

    Google Scholar 

  • Wu W, Zhou R, Huang Y, Boufford DE, Shi S (2010) Molecular evidence for natural intergeneric hybridization between Liquidambar and Altingia. J Plant Res 123:321–239

    Article  Google Scholar 

Download references

Acknowledgments

Arturo Roig-Alsina and Rocío Fernández-Vaquero helped with insect identification. Lone Aagesen provided us with two unpublished trnL sequences of D. chacaye × D. articulata. Alicia Basilio, María Fernanda Reyes, Manuel de Paz, Mariano Devoto, and Norberto H. Montaldo helped with fieldwork. Norberto Bartoloni helped with statistical analyses. Comments by Mariano Devoto and three anonymous reviewers helped to improve a previous draft. Financial support was granted by Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) to D.M. and A.C.P. [PIP 5704].

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Authors and Affiliations

  1. Cátedra de Botánica Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Avda. San Martín 4453, C1417DSQ, Ciudad Autónoma de Buenos Aires, Argentina

    Diego Medan

  2. Universidad Nacional de Río Negro, Sede Andina, 8400, Bariloche, Argentina

    Marina Arbetman

  3. Laboratorio Ecotono, Universidad Nacional del Comahue e INIBIOMA-CONICET, Quintral 1250, 8400, Bariloche, Argentina

    Marina Arbetman, Eugenia E. Chaia & Andrea C. Premoli

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  1. Diego Medan
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  2. Marina Arbetman
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  4. Andrea C. Premoli
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Correspondence to Diego Medan.

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Medan, D., Arbetman, M., Chaia, E.E. et al. Interspecific and intergeneric hybridization in South American Rhamnaceae-Colletieae. Plant Syst Evol 298, 1425–1435 (2012). https://doi.org/10.1007/s00606-012-0646-0

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