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Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of retinol-binding protein 4 in urine

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Abstract

Urine retinol-binding protein 4 (RBP4) has recently been reported as a novel earlier biomarker of chronic kidney disease (CKD) which is a global public health problem with high morbidity and mortality. Accurate and rapid detection of urine RBP4 is essential for early monitor of impaired kidney function and prevention of CKD progression. In the present study, we developed a time-resolved fluorescence immunochromatographic test strip (TRFIS) for the quantitative and rapid detection of urine RBP4. This TRFIS possessed excellent linearity ranging from 0.024 to 12.50 ng/mL for the detection of urine RBP4, and displayed a good linearity (Y = 239,581 × X + 617,238, R2 = 0.9902), with the lowest visual detection limit of 0.049 ng/mL. This TRFIS allows for quantitative detection of urine RBP4 within 15 min and shows high specificity. The intra-batch coefficient of variation (CV) and the inter-batch CV were both < 8%, respectively. Additionally, this TRFIS was applied to detect RBP4 in the urine samples from healthy donors and patients with CKD, and the results of TRFIS could efficiently discern the patients with CKD from the healthy donors. The developed TRFIS has the characteristics of high sensitivity, high accuracy, and a wide linear range, and is suitable for rapid and quantitative determination of urine RBP4.

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Data availability

The datasets and materials used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by the Science and Technology Planning Project of Guangdong Province (No. 2023B1212060062) and the Shenzhen Science and Technology Program (No. JCYJ20220531092201003).

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Author notes

  1. Wenjie Guo and Zhiyong Yu contributed equally to this work.

Authors and Affiliations

  1. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, Guangdong, China

    Wenjie Guo, Zhiyong Yu, Tianxu Li, Huiqi Lin, Ying Liao, Yanghao Zheng & Jie Zan

  2. Department of Nephrology, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518033, Guangdong, China

    Lingfei Lu & Jiandong Lu

  3. The Second Affiliated Hospital of Guangzhou, Guangdong Provincial Key Laboratory of Research on Emergency in TCM, University of Chinese Medicine, Guangzhou, Guangdong, China

    Yuntao Liu & Jie Zan

  4. Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, Guangdong, China

    Jinping Chen

  5. Institute of Experimental Veterinary Medicine named of S.N. Vyshelessky, Minsk, Belarus

    Glaskovich Maria Alevtinovna & Dzmitry S. Barysavets

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  1. Wenjie Guo
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Contributions

Conceptualization, experimental design, data curation, and original draft preparation, WG, ZY, TL, LL, HL, YL, HZ, JZ, and JL; investigation and manuscript review, WG, ZY, TL, LL, HL, YL, YZ, YL, GMA, DSB, JC, JZ, and JL; supervision and funding acquisition, JZ and JL. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Jie Zan or Jiandong Lu.

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The study was approved by institutional ethics board of Shenzhen Traditional Chinese Medicine Hospital (K2022-001-02).

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Guo, W., Yu, Z., Li, T. et al. Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of retinol-binding protein 4 in urine. Microchim Acta 191, 311 (2024). https://doi.org/10.1007/s00604-024-06381-6

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