Abstract
Novel chiral capillary electrochromatography (CEC) microsystems were constructed based on Aspergillus sp. CM96. As a newly discovered intrinsic characteristic of the cell, cell chirality occupies an essential position in life evolution. Aspergillus sp. CM96 spore (CM96s) was chosen as a proof of concept to develop chiral capillary columns. Interestingly, various types of amino acid (AA) enantiomers were baseline separated under the optimized conditions. Furthermore, the time-dependent chiral interactions between AAs and CM96s were explored in a wider space. Pectinases generated from Aspergillus sp. CM96 fermentation were immobilized onto graphene oxide–functionalized capillary silica monoliths for separating AA enantiomers. Molecular docking simulations were performed to explore chiral separation mechanisms of pectinase for AA enantiomers. These results indicated that Aspergillus sp. CM96-based CEC microsystems have a significant advantage for chiral separation.
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For financial support of this work, we acknowledge the National Natural Science Foundation of China (81803495); Natural Science Foundation of Hunan Province (2019JJ50749).
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Tingting Hong and Zhiqiang Cai conceived the project; Tingting Hong and Xing Liu designed and performed the experiments. All the authors discussed the results. Tingting Hong, Yibing Ji, and Songwen Tan wrote the original draft. All authors contributed to finalizing the manuscript.
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Hong, T., Liu, X., Ji, Y. et al. Construction of chiral capillary electrochromatography microsystems based on Aspergillus sp. CM96. Microchim Acta 190, 357 (2023). https://doi.org/10.1007/s00604-023-05926-5
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DOI: https://doi.org/10.1007/s00604-023-05926-5