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Specific capture and determination of glycoprotein using a hybrid epitopes and monomers-mediated molecular-imprinted polymer enzyme-free electrochemical biosensor

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Abstract 

A novel molecular-imprinted polymer (MIP)–based enzyme-free biosensor was created for the selective detection of glycoprotein transferrin (Trf). For this purpose, MIP-based biosensor for Trf was prepared by electrochemical co-polymerization of novel hybrid monomers 3-aminophenylboronic acid (M-APBA) and pyrrole on a glassy carbon electrode (GCE) modified with carboxylated multi-walled carbon nanotubes (cMWCNTs). Hybrid epitopes of Trf (C-terminal fragment and glycan) have been selected as templates. The produced sensor exhibited great selective recognition ability toward Trf under optimal preparation conditions, offering good analytical range (0.125–1.25 μM) with a detection limit of 0.024 μM. The proposed hybrid epitope in combination with hybrid monomer-mediated imprinting strategy was successfully applied to detect Trf in spiked human serum samples, with recoveries and relative standard deviations ranging from 94.7 to 106.0% and 2.64 to 5.32%, respectively. This study provided a reliable protocol for preparing hybrid epitopes and monomers-mediated MIP for the synergistic and effective determination of glycoprotein in complicated biological samples.

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Funding

This work was financially supported by National Natural Science Foundation of China (No.81973451), Fundamental and Frontier Research Fund of Chongqing (No. cstc2018jcyjAX0661, No.cstc2019jsyj-yzysbAX0020), Graduate Research and Innovation Project of Chongqing, 2022 (CYB22041), the Science and Technology Research Program of Chongqing Municipal Education Commission (No. KJZD-K201800103), Fundamental Research Funds for the Central Universities (No. 2019CDYGYB027, 2022CDJXY-003), Graduate Research and Innovation Foundation of Chongqing (No. CYS18033), Venture & Innovation Support Program for Chongqing Overseas Returnees, and Tang Foundations.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China

    Jia-Yuan He, Hui-Xian Xu, Qin Li & Qi-Hui Zhang

  2. Basic Medical College, Chongqing Medical University, Chongqing, 400016, China

    Lian-Di Zhou

  3. Chongqing Cancer Institute, Chongqing University Cancer Hospital, Chongqing, 400030, China

    Ling Luo

  4. Chongqing Institute for Food and Drug Control, Chongqing, 401121, China

    Qin-Yue Zheng

  5. Tang Center for Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, 60637, USA

    Chong-Zhi Wang, Qi-Hui Zhang & Chun-Su Yuan

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  1. Jia-Yuan He
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He, JY., Xu, HX., Li, Q. et al. Specific capture and determination of glycoprotein using a hybrid epitopes and monomers-mediated molecular-imprinted polymer enzyme-free electrochemical biosensor. Microchim Acta 190, 118 (2023). https://doi.org/10.1007/s00604-023-05651-z

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