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In situ vertical alignment of 2D MoS2 layers on GO film: enhanced electrochemical properties for PD-L1 sensing

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Abstract

There is an urgent need for a flexible and simple programmed cell death ligand 1 (PD-L1) dynamic measurement method enabling real-time monitoring of cancer progression and assessment of immunotherapy efficacy. In the current study, we show facile in situ synthesis of vertical alignment two-dimensional molybdenum disulfide (2D MoS2) layers on graphene-oxide-modified ITO (MoS2┴GO-ITO) using a hydrothermal approach and demonstrate the importance of the alignment of 2D in achieving high-probe capturing, enhanced electrochemical properties and target selectivity during sensing. After modification of designed PD-L1 binding peptides on the MoS2┴GO-ITO, a sensitive PD-L1 electrochemical sensor was designed using vertical alignment MoS2 to capture more probes for PD-L1 recognition and excellent in plane electron transport to accelerate electrochemical signals. The fabricated electrochemical sensor could sensitively determine PD-L1 in a wide linear range of 25–500 ng/mL and exhibit desirable accuracy and reliability in clinical samples application. This simple and sensitive method is likely to investigate further research into the exploration of the perpendicular alignment of 2D surfaces for diverse applications.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 61875114 and 62005156).

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Authors and Affiliations

  1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China

    Zhihui Mao

  2. Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, China

    Zhihui Mao, Han Zhu, Jie Chen, Xiaojun Hu & Hongxia Chen

  3. School of Medicine, Shanghai University, Shanghai, 200444, China

    Xinsheng Peng, Jie Chen & Qiang Chen

  4. Department of Oncology, the Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, 450008, China

    XiaoBing Chen

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  1. Zhihui Mao
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Correspondence to Hongxia Chen.

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Mao, Z., Zhu, H., Peng, X. et al. In situ vertical alignment of 2D MoS2 layers on GO film: enhanced electrochemical properties for PD-L1 sensing. Microchim Acta 189, 155 (2022). https://doi.org/10.1007/s00604-022-05269-7

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