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Immobilization of cell membrane onto a glucose-Zn-based porous coordination polymer and its application to rapid screening of potentially active compounds from Vaccinium corymbosum L. leaves

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Abstract

A novel glucose-Zn-based porous coordination polymer (PCP) was selected as a carrier of cell membranes (CMs) to fabricate CM-coated PCP (CMPCP) for rapid screening of potentially active compounds from natural products. The cell disruption and the amount of maximum CMs adsorbed on PCP were optimized according to the amount of immobilized protein. This new kind of matrix exhibited good reproducibility and stability, and was applied for fishing potentially active compounds from the extracts of Vaccinium corymbosum L. leaves (VCL). Using LC-MS/MS, chlorogenic acid and quercetin were identified as the potentially active compounds through comparison of normal and non-alcoholic fatty liver disease (NAFLD)–modeled CMPCP. Our results suggested that the proposed approach based on CMPCP was environmentally friendly, cost-effective, and convenient in terms of green porous material, stable protein loading capacity, and accessible operation process. The developed method could provide a promising platform for efficient drug discovery from natural product resources.

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Funding

This research was funded by the Science and Technology Development Fund, Macau SAR (file nos. 0077/2019/A2 and 0023/2019/AKP).

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Author notes

  1. Yan Li and Yanli Chen contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, 999078, China

    Yan Li, Yanli Chen, Huixia Zhang, Zheng Li, Caiyun Wang, Wei Zhang & Zhihong Jiang

  2. Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau, 999078, China

    Christopher Wai Kei Lam

  3. Tianjin Key Laboratory of Advanced Functional Porous Materials, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin, 300384, China

    Yunfeng Zhao

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  1. Yan Li
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  2. Yanli Chen
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  4. Christopher Wai Kei Lam
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  5. Zheng Li
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Correspondence to Yunfeng Zhao, Wei Zhang or Zhihong Jiang.

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Li, Y., Chen, Y., Zhang, H. et al. Immobilization of cell membrane onto a glucose-Zn-based porous coordination polymer and its application to rapid screening of potentially active compounds from Vaccinium corymbosum L. leaves. Microchim Acta 187, 630 (2020). https://doi.org/10.1007/s00604-020-04612-0

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  • DOI: https://doi.org/10.1007/s00604-020-04612-0

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